Lipids

, Volume 6, Issue 11, pp 805–814 | Cite as

A comparison of triglyceride, monoglyceride, and phospholipid substrates for post-heparin lipolytic activities from normal and hypertriglyceridemic subjects

  • William C. Vogel
  • John D. Brunzell
  • Edwin L. Bierman
Article

Abstract

Evidence for heterogeneity of postheparin lipolytic activities in plasma was explored. After low or high doses of heparin, activities were measured in assays with phospholipid or monoglyceride substrate and with triglyceride substrate in assays using either calcium or albumin as the fatty acid acceptor. An excellent correlation between all assays was obtained in normals after both low and high doses of heparin over a wide range of individual response. In selected subjects with hypertriglyceridemia known to be deficient in response to heparin, all responses measured with phospholipid or triglyceride were below the lower limit of normal response. Some responses with monoglyceride substrate were within the normal range, pathophysiological evidence of a separate monoglyceridase. There was no evidence in either group of separate triglyceride lipase and phospholipase activities. The closest correlation between assays in normals was between triglyceride substrate with calcium as the fatty acid acceptor and the assay with monoglyceride. No differential effect upon lipolytic activities was found (1) after preincubation of the plasma, (2) with inhibitors in the assay, or (3) with preincubation of plasma and inhibitors prior to the assay.

Keywords

Lipase Triolein Protamine Monoglyceride Lipolytic Activity 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Vogel, W.C. and L. Zieve, J. Lipid Res. 5:177–183 (1964).PubMedGoogle Scholar
  2. 2.
    Vogel, W.C., W.G. Ryan, J.L. Koppel and J.H. Olwin, Ibid. 6:335–340 (1965).PubMedGoogle Scholar
  3. 3.
    Vogel, W.C. and E.L. Bierman, Ibid. 8:46–53 (1967).PubMedGoogle Scholar
  4. 4.
    Vogel, W.C. and E.L. Bierman, Proc. Soc. Exp. Biol. Med. 127:77–80 (1968).PubMedGoogle Scholar
  5. 5.
    Vogel, W.C. and E.L. Bierman, Lipids 5:385–391 (1970).PubMedCrossRefGoogle Scholar
  6. 6.
    Biale, Y. and E. Shafrir, Clin. Chim. Acta 23:413–419 (1969).PubMedCrossRefGoogle Scholar
  7. 7.
    Greten, H., R.I. Levy and D.S. Fredrickson, J. Lipid Res. 10:326–330 (1969).PubMedGoogle Scholar
  8. 8.
    Fredrickson, D.S., K. Ono and L.L. Davis, Ibid. 4:24–33 (1963).PubMedGoogle Scholar
  9. 9.
    Hirsch, J. and E.H. Ahrens, Jr., J. Biol. Chem. 233:311–320 (1958).PubMedGoogle Scholar
  10. 10.
    Porte, D., Jr. and E.L. Bierman, J. Lab. Clin. Med. 73:631–648 (1969).PubMedGoogle Scholar
  11. 11.
    Dole, V.P. and H. Meinertz, J. Biol. Chem. 235:2595–2599 (1960).PubMedGoogle Scholar
  12. 12.
    Marinetti, G.V., J. Lipid Res. 6:315–317 (1965).PubMedGoogle Scholar
  13. 13.
    Greten, H., R.I. Levy and D.S. Fredrickson, Biochim. Biophys. Acta 164:185–194 (1968).PubMedGoogle Scholar
  14. 14.
    Boberg, J., Lipids 5:452–456 (1970).PubMedCrossRefGoogle Scholar
  15. 15.
    Datta, D.V., Proc. Soc. Biol. Exp. Biol. Med. 112: 1006–1008 (1963).Google Scholar
  16. 16.
    Hanson, R.W. and F.J. Ballard J. Lipid Res. 9:667–668 (1968).PubMedGoogle Scholar
  17. 17.
    Persson, B. and B. Hood, Atherosclerosis 12:241–251 (1970).PubMedCrossRefGoogle Scholar
  18. 18.
    Shore, B. and V. Shore, Am. J. Physiol. 201:915–922 (1961).PubMedGoogle Scholar
  19. 19.
    Shore, B. and V. Shore, Nature 193:163–164. (1962).PubMedCrossRefGoogle Scholar
  20. 20.
    Korn, E.D., J. Biol. Chem. 215:1–14 (1955).PubMedGoogle Scholar
  21. 21.
    Korn, E.D. and T.W. Quiqley, Jr., J. Biol. Chem. 226:833–839 (1957).PubMedGoogle Scholar
  22. 22.
    Datta, D.V. and H.S. Wiggins, Proc. Soc. Exp. Biol. Med. 115:788–792 (1964).PubMedGoogle Scholar

Copyright information

© American Oil Chemists’ Society 1971

Authors and Affiliations

  • William C. Vogel
    • 1
    • 2
  • John D. Brunzell
    • 1
    • 2
  • Edwin L. Bierman
    • 1
    • 2
  1. 1.Department of MedicineVeterans Administration HospitalSeattle
  2. 2.the University of Washington School of MedicineSeattle

Personalised recommendations