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Cholesterol content of human parotid saliva

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Lipids

Abstract

Free and esterifed cholesterol were determined in parotid fluid collected from 19 individuals by encapsulation of Stenson's duct in the oral 16.7±7.1 (SD) μg/100 ml and cholesterol esters averaged 13.2±8.0 (SD) μg/100 ml. Two assays are described for the measurement of cholesterol in parotid saliva. Lipophilic material was extracted with ether-ethanol (3∶1). In assay 1, the solid residue of the extract was separated into its components by thin layer chromatography. Substances on the chromatogram were made to fluoresce and the spots were photographed. Cholesterol was determined by densitometry of its black image on film. In assay 2, the ether-ethanol residue was subjected to quantitative gas chromatography. Both methods yield similar data.

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Author information

Author notes

  1. Raf Ael J. Montiel

    Present address: Facultad de Medicina, Universidad del Zulia, Maracaibo, Venezuela

Authors and Affiliations

  1. Department of Biological Chemistry, Hahnemann Medical College, 19102, Philadelphia, Pennsylvania

    Gert M. Jacobsohn & Raf Ael J. Montiel

Authors
  1. Gert M. Jacobsohn
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  2. Raf Ael J. Montiel
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Jacobsohn, G.M., Montiel, R.A.J. Cholesterol content of human parotid saliva. Lipids 4, 380–383 (1969). https://doi.org/10.1007/BF02531009

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  • DOI: https://doi.org/10.1007/BF02531009

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