Skip to main content
SpringerLink
Log in

Steroids in bovine muscle and adipose tissue

  • Published:
Lipids

Abstract

Thin layer and gas liquid chromatography, (GLC) were employed as complementary techniques to investigate naturally-occurring steroids in the unsaponifiable matter of bovine muscle and adipose tissue. Three GLC liquid phases, differing in selective partition properties, were used to effectively identify unknown steroids. The results indicate that cholesterol and minor amounts of desmosterol, Δ7-cholestenol, lanosterol, dihydrolanosterol, dehydromethostenol, Δ8-methostenol, Δ7-methostenol, cholestanol and possibly ergosterol were present in the bovine tissues. The minor steroids, with the exception of cholestanol and ergosterol, are steroid precursors in cholesterol biosynthesis. Common hormonal steroids were not found in the unsaponifiables of the tissues.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Watt, B. K., and A. L. Merrill, Agr. Handbook No. 8, USDA, 1963.

  2. Williams, L. D., and A. M. Pearson, J. Agr. Food Chem.13, 573–577 (1965).

    Article  CAS  Google Scholar 

  3. Tu, C., W. D. Powrie, and O. Fennema, J. Food Sci.32, 30–34 (1967).

    Article  CAS  Google Scholar 

  4. Mahesh, V. B., and F. Ulrich, J. Biol. Chem.235, 356–360 (1960).

    PubMed  CAS  Google Scholar 

  5. Sweat, M. L., and M. J. Bryson, Biochim. Biophys. Acta44, 217–223 (1960).

    Article  PubMed  CAS  Google Scholar 

  6. Lipman, M. M., F. H. Katz and J. W. Jailer, J. Clin. Endocrinol. Metab.22, 268–272 (1962).

    Article  PubMed  CAS  Google Scholar 

  7. Thomas, P. Z., and R. I. Dorfman, J. Biol. Chem.239, 762–765 (1964).

    PubMed  CAS  Google Scholar 

  8. Dietschy, J. M., and M. D. Siperstein, J. Lipid Res.8, 97–104 (1967).

    PubMed  CAS  Google Scholar 

  9. Durr, I. F., Biochem. J.98, 317–320 (1966).

    PubMed  CAS  Google Scholar 

  10. Truswell, A. S., and W. D. Mitchell, J. Lipid Res.6, 438–441 (1965).

    PubMed  CAS  Google Scholar 

  11. VandenHeuvel, W. J. A., and E. C. Horning, Biochim. Biophys. Acta, 64, 416–429 (1962).

    Article  PubMed  CAS  Google Scholar 

  12. Chicoye, E., W. D. Powrie, and O. Fennema, Lipids4, 335–339 (1968).

    Google Scholar 

  13. Menge, H., R. J. Lillie, and C. A. Denton, J. Nutr.63, 499–508 (1957).

    PubMed  CAS  Google Scholar 

  14. Clayton, R. B., A. N. Nelson, and I. D. Frantz, Jr., J. Lipid Res.4, 166–176 (1963).

    PubMed  CAS  Google Scholar 

  15. Frantz, I. D., Jr., and G. J. Schroepfer, Jr., Ann. Rev. Biochem.36, 691–726 (1967).

    Article  CAS  PubMed  Google Scholar 

  16. Loud, A. V., and N. L. R. Bucher, J. Biol. Chem.233, 37–41 (1958).

    PubMed  CAS  Google Scholar 

  17. Kandutsch, A. A., and A. E. Russell, Ibid.235, 2256–2261 (1960).

    PubMed  CAS  Google Scholar 

  18. Bloch, K., in “Vitamins and Hormones”, Vol. 15, Editors, R. S. Harris, G. F. Marrian and K. V. Thimann, Academic Press, Inc., New York, 1957, p. 119.

    Google Scholar 

  19. Horlick, L., and J. Avigan, J. Lipid Res.4, 160–165 (1963).

    PubMed  CAS  Google Scholar 

  20. Horlick, L., Ibid.7, 116–121 (1966).

    PubMed  CAS  Google Scholar 

  21. Avigan, J., D. S. Goodman, and D. Steinberg, J. Biol. Chem.238, 1283–1293 (1963).

    PubMed  CAS  Google Scholar 

  22. Goodman, D. S., J. Avigan and D. Steinberg Ibid.238, 1287–1293 (1963).

    PubMed  CAS  Google Scholar 

  23. Olson, J. A., Jr., M. Lindberg, and K. Bloch Ibid.226, 941–953 (1957).

    PubMed  CAS  Google Scholar 

  24. Kandutsch, A. A., and A. E. Russell,Ibid.235, 2253–2255 (1960).

    PubMed  CAS  Google Scholar 

  25. Wells, W. W., and D. H. Neiderhiser, J. Am. Chem. Soc.79, 6569–6570 (1957).

    CAS  Google Scholar 

  26. Stokes, W. M., F. C. Hickey, and W. A. Fish, J. Biol. Chem.232, 347–359 (1958).

    PubMed  CAS  Google Scholar 

  27. Stokes, W. M., W. A. Fish, and F. C. Hickey Ibid.220, 415–430 (1956).

    PubMed  CAS  Google Scholar 

  28. Stokes, W. M., and W. A. Fish, Ibid.235, 2604–2607 (1960).

    CAS  Google Scholar 

  29. Fumagelli, R., R. Niemiro, and R. Paoletti, JAOCS42, 1018–1023 (1965).

    Google Scholar 

  30. Paoletti, R., R. Fumagelli, E. Grossi, and P. Paoletti, Ibid., 400–404 (1965).

    PubMed  CAS  Google Scholar 

  31. Kritchevsky, D., and W. L. Holmes, Biochem. Biophys. Res. Commun.7, 128–131 (1962).

    Article  PubMed  CAS  Google Scholar 

  32. Avigan, J., D. Steinberg, M. J. Thompson, E. Mosettig, Porg. Cardiovascular Diseases2, 525–530 (1960).

    Article  CAS  Google Scholar 

  33. Blohm, T. R., T. Kariya, M. W. Laughlin, Arch. Biochem. Biophys.85, 250–263 (1959).

    Article  PubMed  CAS  Google Scholar 

  34. Mosbach, E. H., J. Blum, E. Arroyo and S. Milch, Anal. Biochem.5, 158–169 (1963).

    Article  PubMed  CAS  Google Scholar 

  35. Werbin, H., I. L. Chaikoff, and M. R. Imada, J. Biol. Chem.237, 2072–2077 (1962).

    PubMed  CAS  Google Scholar 

  36. Sutton, D. A., JAOCS32, 16–22 (1955).

    CAS  Google Scholar 

  37. Shefer, S., S. Milch, and E. H. Mosbach, J. Lipid Res.6, 33–36 (1965).

    PubMed  CAS  Google Scholar 

  38. Rosenfeld, R. S., B. Zumoff, and L. Hellman, Ibid.8, 16–23 (1967).

    PubMed  CAS  Google Scholar 

  39. Shefer, S., S. Hauser, and E. H. Mosbach, Ibid.7, 763–771 (1966).

    PubMed  CAS  Google Scholar 

  40. Pennock, J. F., G. Neiss, and H. R. Mahler, Biochem. J.85, 530–537 (1962).

    PubMed  CAS  Google Scholar 

  41. Kochakian, C. D., and G. Stidworthy, J. Biol. Chem.199, 607–612 (1952).

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Food Science, University of Wisconsin, 53706, Madison, Wisconsin

    C. Tu (Postdoctoral research associate), W. D. Powrie & O. Fennema

Authors
  1. C. Tu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. W. D. Powrie
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. O. Fennema
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Tu, C., Powrie, W.D. & Fennema, O. Steroids in bovine muscle and adipose tissue. Lipids 4, 369–379 (1969). https://doi.org/10.1007/BF02531008

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02531008

Keywords

Search

Navigation