, Volume 3, Issue 1, pp 84–87 | Cite as

Effects of experimental endogenous hyperlipemia on circulating leukocytes and erythrocytes

  • L. Allen Ehrhart
  • Antanas Butkus
  • A. Lazzarini RobertsonJr.
  • Irvine H. Page


Effects of hyperlipemia on circulating leukocytes and erythrocytes were studied in dogs which were given repeated, intravenous injections of a nonionic detergent, Triton WR-1339.

Erythrocyte lipid concentrations increased from 3.6±0.9 mg/1010 cells in control animals to 9.3±1.5 mg in the hyperlipemic group. This increase was accompanied by a shift in the distribution of total fatty acids toward a higher percentage of saturated and monounsaturated acids. In contrast to the changes observed in erythrocytes, the leukocyte lipid content remained unaltered in dogs with serum cholesterol levels ranging from 500 to more than 2,000 mg/100 ml. Leukocyte counts rose whereas hematocrit values, hemoglobin concentrations, and erythrocyte counts decreased. Oxygen utilization studies showed no significant metabolic differences between leukocytes which were isolated from hyperlipemic or control animals. Circulating leukocytes in dogs with an endogenously induced hyperlipemia were shown therefore to maintain normal lipid concentrations and did not participate in lipophage formation, as reported for certain diet-induced lipemias.


Nile Blue Erythrocyte Count Fatty Acid Distribution Differential Leukocyte Count Leukocyte Suspension 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Geer, J. C., Lab. Invest.14, 1764–1783 (1965).PubMedGoogle Scholar
  2. 2.
    Still, W. J. S., and P. R. Marriott, J. Atherosclerosis Res.4, 373–386 (1964).Google Scholar
  3. 3.
    Suzuki, M., S. D. Greenberg, J. G. Adams and R. M. O'Neal, Exptl. Mol. Pathol.,3, 455–67 (1964).CrossRefGoogle Scholar
  4. 4.
    Geer, J. C., Am. J. Pathol.,47, 241–269 (1965).PubMedGoogle Scholar
  5. 5.
    Still, W. J. S., and R. M. O'Neal, Ibid.40, 21–35 (1962).PubMedGoogle Scholar
  6. 6.
    Simon, R. C., W. J. S. Still and R. M. O'Neal, J. Atherosclerosis Res.1, 395–400 (1961).CrossRefGoogle Scholar
  7. 7.
    Skoog, W. A., and W. S. Beck, Blood,11, 436–454 (1956).PubMedGoogle Scholar
  8. 8.
    Folch, J., M. Lees and G. H. Sloane Stanley, J. Biol. Chem.226, 497–509 (1957).PubMedGoogle Scholar
  9. 9.
    Butkus, A., and J. N. Berretoni, Lipids2, 212–216 (1967).PubMedGoogle Scholar
  10. 10.
    Adams, C. W. M., J. Histochem. Cytochem.8, 262–267 (1960).PubMedGoogle Scholar
  11. 11.
    Suzuki, M., and R. M. O'Neal, J. Lipid Res.5, 624–627 (1964).PubMedGoogle Scholar
  12. 12.
    Suzuki, M., and R. M. O'Neal, Arch. Pathol.83, 169–174 (1967).PubMedGoogle Scholar
  13. 13.
    Butkus, A., A. L. Robertson, Jr., L. A. Ehrhart and I. H. Page, J. Atherosclerosis Res., in press.Google Scholar

Copyright information

© American Oil Chemists’ Society 1968

Authors and Affiliations

  • L. Allen Ehrhart
    • 1
  • Antanas Butkus
    • 1
  • A. Lazzarini RobertsonJr.
    • 1
  • Irvine H. Page
    • 1
  1. 1.Research DivisionThe Cleveland Clinic FoundationCleveland

Personalised recommendations