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Archives of Microbiology

, Volume 164, Issue 6, pp 414–419 | Cite as

The α-d-mannan core of a complex cell-wall heteroglycan ofTrichoderma reesei is responsible for β-glucosidase activation

  • Johannes Rath
  • Robert Messner
  • Paul Kosma
  • Friedrich Altmann
  • Leopold März
  • Christian P. Kubicek
Original Paper
  • 45 Downloads

Abstract

A heteroglycan responsible for the binding of the enzyme β-1,4-d-glucosidase (EC 3.2.1.21) to fungal cell walls was isolated from cell walls of the filamentous fungusTrichoderma reesei. The heteroglycan, composed of mannose, galactose, glucose, and glucuronic acid, also activated β-1,4-d-glucosidase, β-1,4-d-xylosidase andN-acetyl-β-1,4-d-glucosaminidase activity in vitro. The structural backbone of this heteroglycan was prepared by acid hydrolysis and gel filtration. The molecular structure of the core of the heteroglycan was determined by NMR studies as a linear α-1,6-d-mannan. The mannan core obtained by acid degradation stimulated the β-glucosidase activity by 90%. Several glycosidases fromAspergillus niger were also activated by theT. reesei heteroglycan. The β-glucosidase ofTrichoderma was activated by mannan fromSaccharomyces cerevisiae to a comparable extent.

Key words

Heteroglycan Cell walls 1,6-α-d-Mannan Trichoderma Aspergillus β-Glucosidase Glycosidases 

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Johannes Rath
    • 1
  • Robert Messner
    • 2
  • Paul Kosma
    • 3
  • Friedrich Altmann
    • 3
  • Leopold März
    • 3
  • Christian P. Kubicek
    • 4
  1. 1.Zentrallabor für Molekularbiologie, Institut für ZoologieUniversität WienWienAustria
  2. 2.Institut für Angewandte MikrobiologieUniversität für BodenkulturWienAustria
  3. 3.Institut für ChemieUniversität für BodenkulturWienAustria
  4. 4.Abteilung für Mikrobielle Biochemie, Institut für Biochemische Technologie und MikrobiologieTU WienWienAustria

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