Abstract
A high copy, tandemly repeated, sequence (Bd49) specific to the B chromosome and located near the centromere inBrachycome dichromosomatica was used to identify lambda genomic clones from DNA of a 3B plant. Only one clone of those analysed was composed entirely of a tandem array of the B-specific repeat unit. In other clones, the Bd49 repeats were linked to, or interspersed with, sequences that are repetitious and distributed elsewhere on the A and B chromosomes. One such repetitious flanking sequence has similarity to retrotransposon sequences and a second is similar to chloroplast DNA sequences. Of the four separate junctions analysed of Bd49-like sequence with flanking sequence, three were associated with the same A/T-rich region in Bd49 and the fourth was close to a 25 bp imperfect dyadic sequence. No novel B-specific sequences were detected within the genomic clones.
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Franks, T.K., Houben, A., Leach, C.R. et al. The molecular organisation of a B chromosome tandem repeat sequence fromBrachycome dichromosomatica . Chromosoma 105, 223–230 (1996). https://doi.org/10.1007/BF02528770
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DOI: https://doi.org/10.1007/BF02528770