Neurochemical Research

, Volume 21, Issue 1, pp 41–46 | Cite as

Cyclic GMP-phosphodiesterase inhibition does not alter cerebral oxygen consumption

  • Hwu Meei Wei
  • Abhijit J. Shah
  • James Tse
  • Oak Za Chi
  • Harvey R. Weiss
Article

Abstract

The effect of zaprinast, a cyclic guanosine monophosphate inhibitor, on the level of cyclic GMP and cerebral O2 consumption was determined. Anesthetized male Long-Evans rats were divided into a control group (n=15) and a zaprinast treated group (n=15). Vehicle was applied topically to the left cortex and 3·10−3 M zaprinast was applied to the right cortex. A saline treated control group was also studied. Regional cerebral blood flow was determined by [14C]-iodoantipyrine and regional O2 extraction was determined by microspectrophotometry. The level of cyclic GMP was measured by radioimmunoassay. There were no hemodynamic or blood gas differences between groups. The level of cyclic GMP was not significantly different between the right and left cerebral cortex of the control group (17.0±4.3 and 17.7±4.6 pmol/g). In the zaprinast treated group, there was a significant (46%) increase in the level of cyclic GMP in the zaprinast treated cortex (20.5±8.1) in comparison to the vehicle treated cortex (14.0±5.7). Zaprinast did not significantly alter cerebral blood flow. There were no significant differences in regional O2 extraction. The O2 consumption of the zaprinast treated cortex (8.0±3.3 ml O2·min−1·100 g−1) was not different from that of the vehicle treated cortex (7.0±2.9) or those of the control group. Thus, our data indicated that the increased level of cyclic GMP had no significant effect on cerebral oxygen consumption.

Key Words

Cyclic GMP-phosphodiesterase zaprinast cyclic GMP cerebral blood flow cerebral O2 consumption 

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Copyright information

© Plenum Publishing Corporation 1996

Authors and Affiliations

  • Hwu Meei Wei
    • 1
  • Abhijit J. Shah
    • 1
  • James Tse
    • 1
  • Oak Za Chi
    • 1
  • Harvey R. Weiss
    • 1
  1. 1.Heart and Brain Circulation Laboratory, Department of Physiology and BiophysicsUMDNJ-Robert Wood Johnson Medical SchoolPiscataway

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