Neurochemical Research

, Volume 21, Issue 1, pp 27–33 | Cite as

Enhanced lipid peroxidation by extracellular ATP in PC12 cells

  • Y. Cheng
  • M. Chen
  • M. James-Kracke
  • P. Wixom
  • A. Y. Sun


Recently we have demonstrated that extracellular ATP acts as an excitatory neurotransmitter and enhances cell death in the presence of ferrous ions. By using a newly developed cis-parinaric acid fluorescence technique, we demonstrated that ATP, in a dose dependent manner, enhanced the increased membrane lipid peroxidation in PC12 cells when cells were incubated with micromolar FeCl2/DTP. P2 purinoceptor agonists, α,β-methylene ATP and 2-methylthio-ATP, induced PC12 cell lipid peroxidation, but to a lesser extent than ATP. ATP-induced Ca2+ influx via P2 purinoceptor activation significantly increased the intracellular Ca2+ concentration, which may have triggered a free radical generating cascade(s), and led to membrane lipid peroxidation and cell death. Since oxidative stress has been implicated in certain neurodegenerative diseases such as aging, extracellular ATP may contribute to neuronal cell death by an oxidative mechanism involving lipid peroxidation.

Key words

cis-Parinaric acid lipid peroxidation PC12 cells purinoceptor extracellular ATP reactive oxygen intermediates 

Abbreviations used


α,β-methylene ATP




diethylenetramine pentaacetic acid


Reactive Blue 2


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Copyright information

© Plenum Publishing Corporation 1996

Authors and Affiliations

  • Y. Cheng
    • 1
  • M. Chen
    • 1
  • M. James-Kracke
    • 1
  • P. Wixom
    • 1
  • A. Y. Sun
    • 1
  1. 1.Department of PharmacologyUniversity of MissouriColumbia

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