Abstract
Corynebacterium glutamicum took up glutamine by a sodium-dependent secondary transport system. Both the membrane potential and the sodium gradient were driving forces. Glutamine uptake showed Michaelis-Menten kinetics, with aK m of 36 μM and aV max of 12.5 nmol min−1 (mg dry weight)−1 at pH 7. Despite a pH optimum in the alkaline range around pH 9, it was shown that uncharged glutamine is the transported species. The affinity for the cotransported sodium was relatively low; an apparentK m of 1.4 mM was determined. Among various substrates tested, only asparagine, when added in 50-fold excess, led to an inhibition of glutamine transport. It was concluded that glutamine uptake occurs via a specific transport system in symport with at least one sodium ion.
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Abbreviations
- CCCP :
-
Carbonylcyanide
- m :
-
chlorophenylhydrazone
- ΔΨ:
-
Membrane potential
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Siewe, R.M., Weil, B. & Krämer, R. Glutamine uptake by a sodium-dependent secondary transport system inCorynebacterium glutamicum . Arch. Microbiol. 164, 98–103 (1995). https://doi.org/10.1007/BF02525314
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DOI: https://doi.org/10.1007/BF02525314