Skip to main content
Log in

Lumazine-like fluorescence in a mass of spores of the cellular slime mold,Dictyostelium discoideum

  • Short Communication
  • Published:
Journal of Plant Research Aims and scope Submit manuscript

Abstract

Using a fluorospectrophotometer, we examined the fluorescence of a crude preparation from the spore masses ofDictyostelium discoideum. Fluorescence emission spectra and excitation spectra suggested that the fluorescence of the crude preparation was a lumazine-like fluorescence rather than a pterin-like fluorescence. By using a microspectrophotometer, we observedin situ the fluorescence emission of a lumazine-like substance localized only in the spore mass of the fruiting body.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  • Bonner, J.T. 1947. Evidence for the formation of cell aggregates by chemotaxis in the development of the slime moldDictyostellium discoideum. J. Exp. Zool.106: 1–26.

    Article  CAS  Google Scholar 

  • Bonner, J.T. 1967. Growth on solid media.In The Cellular Slime Mold, 2nd edition, Princeton University Press, New Jersey, pp. 81–86.

    Google Scholar 

  • Gerisch, G., Blank, G., Schweiger, M., Fuchs, D., Hausen, A., Reibneger, G. andWachter, H. 1982. Pteridines released fromDictyostelium discoideum cells into the extracellular medium.In H. Wachter, H.C. Curtius and W. Pfleiderer, eds., Biochemical and Clinical Aspects of Pteridines, vol. 1, Walter de Gruyter and Co., Berlin, pp. 253–256.

    Google Scholar 

  • Klein, R. 1992. Determination of the stereoconfiguration of natural pterin by chiral high-performance liquid chromatography. Anal. Biochem.203: 134–140.

    Article  PubMed  CAS  Google Scholar 

  • Klein, R., Tatischeff, I., Tham, G. andMano, N. 1994. Chiral lumazines: Preparation, properties, enatiomeric separation. Chirality6: 564–571.

    Article  PubMed  CAS  Google Scholar 

  • Klein, R., Thiery, R. andTatischeff, I. 1990. Dictyopterin, 6-(D-threo-1, 2-dihydroxypropyl)-pterin, a new natural isomer of L-biopterin. Isolation from vegetative cells ofDictyostelium discoideum and identification. Eur. J. Biochem.187: 665–669.

    Article  PubMed  CAS  Google Scholar 

  • Schmidt, W., Galland, P., Senger, H. andFuruya, M. 1990. Microspectrophotometry ofEuglena gracilis. Pterin-and flavin-like fluorescence in the paraflagellar body. Planta182: 375–381.

    Article  CAS  Google Scholar 

  • Tatischeff, I. andKlein, R. 1982. Fluorescent products secreted by Dictyostelium discoideum cells which are able to aggregate. FEBS Lett.138: 265–269.

    Article  CAS  Google Scholar 

  • Tatischeff, I., Klein, R. andTham, G. 1984a. Extracellular lumazine from aggregatingDictyostelium discoideum cells. Influence of pH on its fluorescence. Hoppe-Seyler's Z. Physiol. Chem.365: 1255–1262.

    PubMed  CAS  Google Scholar 

  • Tatischeff, I., Klein, R. andTham, G. 1984b. Lumazine formation in the extracellular medium of aggregatingDictyostelium discoideum AX-2 cells.In W. Pfleiderer, H. Wachter and H.C. Curtius, eds., Biochemical and Clinical Aspects of Pteridines, vol. 3, Walter de Gruyter and Co., Berlin, pp. 171–182.

    Google Scholar 

  • Uchiyama, S., Nagai, S. andMaruyama, K. 1993. Localization of fluorescent substances in the cellular slime moldDictyostelium discoideum cells during growth and development. J. Plant Res.106: 345–349.

    Article  Google Scholar 

  • Uchiyama, S., Nagai, S. andMaruyama, K. 1994. Fluorescence in the migrating pseudoplasmodium of the cellular slime moldDictyostelium mucoroides. Cell Struct. Func.19: 159–163.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Uchiyama, S., Nagai, Si. & Maruyama, K. Lumazine-like fluorescence in a mass of spores of the cellular slime mold,Dictyostelium discoideum . J. Plant Res. 110, 383–386 (1997). https://doi.org/10.1007/BF02524938

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02524938

Key words

Navigation