Journal of Plant Research

, Volume 110, Issue 3, pp 305–309 | Cite as

Highly sensitive analytical method for aluminum movement in soybean root through lumogallion staining

  • Tatsuhiko Kataoka
  • Maiko Mori
  • Tomoko M. Nakanishi
  • Satoshi Matsumoto
  • Akira Uchiumi
Original Articles

Abstract

We present highly sensitive aluminum detection method in root using fluorescent lumogallion. Roots treated with 100 μM AlCl3 including 0.2 mM CaCl2 (pH 4.5) were stained for 60 min with 10 μM lumogallion fluorescence solution and fluorescence from aluminum complex in root was observed under confocal laser microscope. There was a good correlation between the intensity of fluorescence and aluminum content. When the amount of aluminum lost during each step in staining process was measured, it was found that about 10% of aluminum was lost only at staining stage. Through lumogallion staining method, aluminum accumulation especially at an early stage of aluminum treatment in root was shown. At the beginning (2 hr), aluminum began to be accumulated in root cap. After 4 hr treatment, the aluminum distribution was spread to about 3 mm from root apex in the root cap and outer cortex. When aluminum was found in the outer cortex in 3–5 mm from the root apex, the viability was tended to be decreased in the same area (6 hr). At the same time, aluminum amount in meristem was increased. However the comparison of lumogallion staining method with that of morin, which has been widely used to detect aluminum in root, the sensitivity of lumogallion method was found to be much higher.

Key words

Aluminum Confocal laser microscope Lumogallion Soybean Staining 

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Copyright information

© The Botanical Society of Japan 1997

Authors and Affiliations

  • Tatsuhiko Kataoka
    • 1
  • Maiko Mori
    • 1
  • Tomoko M. Nakanishi
    • 1
  • Satoshi Matsumoto
    • 1
  • Akira Uchiumi
    • 2
  1. 1.Department of Applied Biological Chemistry, Graduate School of Agricultural and Life SciencesThe University of TokyoTokyoJapan
  2. 2.Department of Analytical ChemistryNational Institute of Materials and Chemical ResearchTsukuba-shi, IbarakiJapan

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