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Modification of membrane fatty acid composition, eicosanoid production, and phospholipase a activity in Atlantic Salmon (Salmo salar) gill and kidney by dietary lipid

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Lipids

Abstract

Atlantic salmon post-smolts were fed diets containing either fish oils (Fosol, FO and Marinol, MO) rich in long-chain n-3 polyunsaturated fatty acids (PUFA), or plant oils rich in 18:2n-6 (sunflower oil, SO) or 18:3n-3 (linseed oil, LO) for 12 wk. The major PUFA in individual phospholipids from gill and kidney were related to the dietary lipid intake. Levels of n-6 PUFA were highest while levels of n-3 PUFA were lowest in fish fed SO. Fish fed LO generally had lower levels of 20:4n-6 compared to the other treatments while fish fed SO generally had the highest levels of 20:4n-6. In all phospholipid classes except phosphatidylinositol (PI) 20:5n-3 was greatest in fish fed MO followed by FO, LO, and SO. In PI, 20:5n-3 was also highest in fish fed MO but those fed LO contained more 20:5n-3 than those fed FO. This resulted in the ratio of the eicosanoid precursors, 20:4n-6/20:5n-3, being significantly greater in fish fed SO, for all phospholipid classes, compared to fish fed the other three dietary oils. The activity of gill phospholipase A was greatest in fish fed FO and was lowest in fish fed SO. The concentration of PGF3α was significantly increased in gill homogenates from fish fed MO compared to the other three treatments while PGF2α was significantly increased in fish fed SO compared to those fed LO. The concentration of PGE3 was significantly reduced in kidney homogenates from fish fed SO compared to the other three treatments while PGE2 was significantly increased in fish fed SO compared to those fed either FO or LO.

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Abbreviations

AA:

arachidonic acid

ANOVA:

analysis of variance

DHA:

docosahexaenoic acid

EPA:

eicosapentaenoic acid

FO:

Fosol fish oil

HETE:

hydroxy eicosatetraenoic acid

HPTLC:

high-performance liquid chromatography

LO:

linseed oil

MO:

Marinol fish oil

PC:

phosphatidylcholine

PE:

phosphatidylethanolamine

PGE:

prostaglanding E

PGF:

prostaglandin F

PI:

phosphatidylinositol

PS:

phosphatidylserine

PUFA:

polyunsaturated fatty acid

SO:

sunflower oil

TLC:

thin-layer chromatography

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Authors and Affiliations

  1. N.E.R.C. Unit of Aquatic Biochemistry, Department of Biological and Molecular Science, University of Stirling, FK9 4LA, Scotland, Stirling, UK

    J. Gordon Bell, Bruce M. Farndale, James R. Dick & John R. Sargent

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  1. J. Gordon Bell
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  2. Bruce M. Farndale
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  3. James R. Dick
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Bell, J.G., Farndale, B.M., Dick, J.R. et al. Modification of membrane fatty acid composition, eicosanoid production, and phospholipase a activity in Atlantic Salmon (Salmo salar) gill and kidney by dietary lipid. Lipids 31, 1163–1171 (1996). https://doi.org/10.1007/BF02524291

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  • DOI: https://doi.org/10.1007/BF02524291

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