Phloem loading in the sucrose-export-defective (SXD-1) mutant maize is limited by callose deposition at plasmodesmata in bundle sheath—vascular parenchyma interface
- 344 Downloads
Using Lucifer Yellow we have demonstrated that the phloem-loading pathway from the mesophyll to the bundle sheath—vascular parenchyma interface inZea mays source leaves follows a symplasmic route in small and intermediate vascular bundles in control as well as in the green sections of mutant sucrose-export-defective (SXD-1) plants. In the anthocyanin-rich mutant leaf sections, Lucifer Yellow transport was prohibited along the same path, at the bundle sheath—vascular parenchyma interface in particular. Plasmodesmata at the latter interface in SXD-1 anthocyanin-rich leaf sections appear to be structurally altered through callose deposition at the plasmodesmal orifices. We suggest that a transport bottleneck at the bundle sheath—vascular parenchyma interface is thus orchestrated and regulated through callose formation, preventing symplasmic transport across this important loading interface.
KeywordsCallose Lucifer yellow Plasmodesma Phloem loading Sucrose-export-defective mutant Zea mays
Unable to display preview. Download preview PDF.
- Apitius A, Lehmann H (1995) The deposition of different wall materials as a wound reaction in the liverwortRiella helicophylla. Cryptogam Bot 5: 351–359Google Scholar
- Goodrick BJ, Kuhn CW, Hussey RS (1991) Restricted systemic movement of cowpea chlorotic mottle virus in soybean with non-necrotic resistance. Phytopathology 81: 1426–1431Google Scholar
- Hughes JE, Gunning BES (1980) Glutaraldehyde-induced deposition of callose. Can J Bot 58: 250–258Google Scholar
- —, Prior DAM, Oparka KJ, Knoblauch M, van Bel AJE (1999) Integration of controlled intercellular pressure injection, iontophoresis and membrane potential measurement. Plant Biol 1: 61–67Google Scholar
- Kollmann R, Glockmann C (1999) Multimorphology and nomenclature of plasmodesmata in higher plants. In: van Bel AJE, van Kesteren WJP (eds) Plasmodesmata: structure, function, role in cell communication. Springer, Berlin Heidelberg New York Tokyo, pp 149–172Google Scholar
- Schulz A (1999) Physiological control of plasmodesmal gating. In: van Bel AJE, van Kesteren WJP (eds) Plasmodesmata: structure, function, role in cell communication. Springer, Berlin Heidelberg New York Tokyo, pp 173–204Google Scholar
- Thompson JR, Garcia-Arenal F (1998) The bundle-sheath phloem interface ofCucumis sativus is a boundary to systemic infection by tomato aspermy virus. Mol Plant Microbe Interact 11: 109–114Google Scholar
- van Bel AJE (1992) Different phloem-loading machineries correlated with the climate. Acta Bot Neerl 41: 121–141Google Scholar