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Journal of the American Oil Chemists’ Society

, Volume 73, Issue 11, pp 1475–1479 | Cite as

Sugar ester-modified lipase for the esterification of fatty acids and long-chain alcohols

  • Sobhi Basheer
  • Mitsutoshi Nakajima
  • Uri Cogan
Biocatalysis Articles

Abstract

The esterification reaction of a long-chain fatty acid and a fatty alcohol with a surfactant-modified lipase in a microaqueousn-hexane system was studied. Various lipases from different sources were first modified with a surfactant of the sugar ester type to improve their dispersibility in apolar organic solvents. This enzyme modification technique converted inactive crude lipases to highly active biocatalysts for the esterification of long-chain fatty acids and fatty alcohols in a microaqueous n-hexane system. The hydrophilic-lipophilic balance value and chainlength of the fatty acid residue of the fatty acid sugar ester, used for modifying the lipases, significantly influenced the amount of precipitated lipase that was dissolved in the aqueous solution, the protein content of the lipase-surfactant complex and its esterification activity.

Key Words

Esterification fatty acids fatty alcohols HLB hydrophilic-lipophilic balance long-chain fatty acid esters microaqueous organic media surfactant-modified lipase 

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Copyright information

© AOCS Press 1996

Authors and Affiliations

  • Sobhi Basheer
    • 1
  • Mitsutoshi Nakajima
    • 2
  • Uri Cogan
    • 1
  1. 1.Department of Food Engineering and BiotechnologyTechnion-Israel Institute of TechnologyHaifaIsrael
  2. 2.National Food Research Institute, Ministry of AgricultureForestry and FisheriesTsukuba, IbarakiJapan

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