Sugar ester-modified lipase for the esterification of fatty acids and long-chain alcohols
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The esterification reaction of a long-chain fatty acid and a fatty alcohol with a surfactant-modified lipase in a microaqueousn-hexane system was studied. Various lipases from different sources were first modified with a surfactant of the sugar ester type to improve their dispersibility in apolar organic solvents. This enzyme modification technique converted inactive crude lipases to highly active biocatalysts for the esterification of long-chain fatty acids and fatty alcohols in a microaqueous n-hexane system. The hydrophilic-lipophilic balance value and chainlength of the fatty acid residue of the fatty acid sugar ester, used for modifying the lipases, significantly influenced the amount of precipitated lipase that was dissolved in the aqueous solution, the protein content of the lipase-surfactant complex and its esterification activity.
Key WordsEsterification fatty acids fatty alcohols HLB hydrophilic-lipophilic balance long-chain fatty acid esters microaqueous organic media surfactant-modified lipase
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- 1.Macrae, A.R., Lipase-Catalyzed Interesterification of Oils and Fats,J. Am. Oil Chem. Soc. 60:291–294 (1983).Google Scholar
- 3.Malcata, F.X., H.R. Reyes, H.S. Garcia, C.G. Hill, and C.H. Amundson Jr., Immobilized Lipase Reactors for Modification of Fats and Oils,J. Am. Oil Chem. Soc. 67:890–910 (1990).Google Scholar
- 4.Sonnet, P.E., G.P. McNeill, and W. Jun, Lipase ofGeotrichum candidum Immobilized on Silica Gel,71:1421–1423 (1994).Google Scholar
- 7.Baillargeon, M.W., and P.E. Sonnet, Polyethylene Glycol Modification ofCandidum rugosa Lipase,J. Am. Oil Chem. Soc. 65:1812–1815 (1988).Google Scholar
- 8.Basri, M. K. Ampon, W.M.Z. Yunus, C.N.A. Razak, and A.B. Salleh, Amidation of Lipase with Hydrophobic Imidoesters,69:579–583 (1992).Google Scholar
- 10.Okahata, Y., and K. Ijiro, A Lipid-Coated Lipase as a New Catalyst for Triglycerides Synthesis in Organic Solvents,J. Chem. Soc., Chem. Commun., 1392–1394 (1988).Google Scholar
- 12.Basheer, S., J.B. Snape, K. Mogi, and M. Nakajima, Transesterification Kinetics of Triglycerides for a Modified Lipase inn-Hexane,J. Am. Oil Chem. Soc. 72:231–237 (1995).Google Scholar
- 13.Basheer, S., K. Mogi, and M. Nakajima, Interesterification Kinetics of Triglycerides and Fatty Acids with Modified Lipase inn-Hexane,72:511–518 (1995).Google Scholar
- 15.Lowry, O.H., N.J. Rosebrough, A.L. Farr, and R.Y.J. Randall, Protein Measurement with the Folin Phenol Reagent,J. Biol. Chem. 193:265–268 (1951).Google Scholar
- 16.Trani, M., F. Ergan, and G. André, Lipase-Catalyzed Production of Wax Esters,J. Am. Oil Chem. Soc. 68:20–22 (1991).Google Scholar
- 17.Boyer, J.L., B. Gilot, and R. Guiraud, Heterogeneous Enzymatic Esterification: Analysis of the Effect of Water,Appl. Microbiol. Biotechnol. 3:372–376 (1990).Google Scholar