Biocatalytical synthesis and monolayer studies of multiple hydroxylated wax esters
The continuous production of aleuritic acid decyl ester has been investigated with LipozymeTM as biocatalyst in a fixed-bed reactor. To avoid the limiting effects of mean substrate solubility, the direct synthesis without organic solvents was chosen, whereby a yield of 90% was obtained depending on the residence time. A comparison of different reaction paths to synthesize a centrally dihydroxylated decyl ester indicated a preference for transesterification of the methyl ester. Under external compression, the interfacial behavior of several enzymatically produced hydroxy wax esters was determined with a Langmuir film balance to find a structure/efficiency relationship. Brewster-angle microscopy allowed direct visualization of a wax ester monolayer at the air/water interface.
Key Wordsπ A-isotherms aleuritic acid biocatalysis Brewster-angle microscopy enzyme fixed-bed reactor esterification hydroxy was esters Langmuir film balance lipase transesterification
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