Journal of the American Oil Chemists’ Society

, Volume 73, Issue 11, pp 1459–1464 | Cite as

Biocatalytical synthesis and monolayer studies of multiple hydroxylated wax esters

  • Wolfram Lokotsch
  • Siegmund Lang
  • Dietmar Möbius
  • Fritz Wagner
Biocatalysis Articles

Abstract

The continuous production of aleuritic acid decyl ester has been investigated with LipozymeTM as biocatalyst in a fixed-bed reactor. To avoid the limiting effects of mean substrate solubility, the direct synthesis without organic solvents was chosen, whereby a yield of 90% was obtained depending on the residence time. A comparison of different reaction paths to synthesize a centrally dihydroxylated decyl ester indicated a preference for transesterification of the methyl ester. Under external compression, the interfacial behavior of several enzymatically produced hydroxy wax esters was determined with a Langmuir film balance to find a structure/efficiency relationship. Brewster-angle microscopy allowed direct visualization of a wax ester monolayer at the air/water interface.

Key Words

π A-isotherms aleuritic acid biocatalysis Brewster-angle microscopy enzyme fixed-bed reactor esterification hydroxy was esters Langmuir film balance lipase transesterification 

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Copyright information

© AOCS Press 1996

Authors and Affiliations

  • Wolfram Lokotsch
    • 2
  • Siegmund Lang
    • 2
  • Dietmar Möbius
    • 1
  • Fritz Wagner
    • 2
  1. 1.Max-Planck-Institut für Biophysikalische ChemieGöttingenGermany
  2. 2.Institut für Biochemie und BiotechnologieTechnische Universität BraunschweigBraunschweigGermany

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