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Lipids

, Volume 31, Issue 4, pp 445–447 | Cite as

Identification of a novel all-cis-5,9,12-Heptadecatrienoic acid in the cellular slime moldPolysphondylium pallidum

  • Tamao Saito
  • Hiroshi Ochiai
Communication

Abstract

The all-cis-5,9,12-heptadecatrienoic acid was identified in the cellular slime moldPolysphondylium pallidum. The structural elucidation was accomplished by capillary gas chromatography, argentation thin-layer chromatography, and gas chromatography/mass spectrometry. This fatty acid has not been reported previously.

Keywords

Fatty Acid Methyl Ester DMDS Dimethyl Disulfide Pyrrolidides Cellular Slime 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

DMDS

dimethyl disulfide

FAME

fatty acid methyl esters

TLC

thin-layer chromatography

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References

  1. 1.
    Long, B.H., and Coe, E.L. (1976) Fatty Acid Compositions of Lipid Fractions from Vegetative Cells and Mature Sorocarps of the Cellular Slime MoldDictyostelium discoideum, Lipids 12, 414–417.Google Scholar
  2. 2.
    Weeks, G., and Herring, F.G. (1980) The Lipid Composition and Membrane Fluidity ofDictyostelium discoideum Plasma Membrane at Various Stages During Differentiation,J. Lipid Res. 21, 681–686.PubMedGoogle Scholar
  3. 3.
    Herring, F.G., Tatischeff, I., and Weeks, G. (1980) The Fluidity of Plasma Membranes ofDictyostelium discoideum. The Effects of Polyunsaturated Fatty Acid Incorporation Assessed by Fluorescence Depolarization and Electronparamagnetic Resonance,Biochim. Biophys. Acta 602, 1–9.PubMedCrossRefGoogle Scholar
  4. 4.
    Manabe, R., Manabe, N., and Ochiai, H. (1990) Monoclonal Antibodies Against the Mercaptoethanol-Sensitive Structure of the Cell-Cell Adhesion Protein ofPolysphondylium pallidum.J. Biochem. (Tokyo) 180, 852–858.Google Scholar
  5. 5.
    Bligh, E.G., and Dyer, W.J. (1959) A Rapid Method of Total Lipid Extraction and Purification,Can. J. Biochem. Physiol. 37, 911–917.PubMedGoogle Scholar
  6. 6.
    Saito, T., and Ochiai, H. (1993) Evidence for a Glycolipid Anchor of gp64, a Putative Cell-Cell Adhesion Protein ofPolysphondylium pallidum, Eur. J. Biochem. 218, 623–628.PubMedCrossRefGoogle Scholar
  7. 7.
    Anderson, B.A., and Holman, R.T. (1974) Pyrrolidides for Mass Spectrometric Determination of the Position of the Double Bond in Monosaturated Fatty Acids,Lipids 9, 185–190.Google Scholar
  8. 8.
    Yamamoto, K., Shibahara, A., Nakayama, T., and Kajimoto, G. (1991) Double-Bond Localization in Heneicosapentaenoic Acid by a Gas Chromatography/Mass Spectrometry (GC/MS) Method,Lipids 26, 948–950.Google Scholar
  9. 9.
    Shibahara, A., Yamamoto, K., Nakayama, T., and Kajimoto, G. (1985) Rapid Determination of Double Bond Positions in Monosaturated Fatty Acids by GC/MS and Its Application to Fatty Acid Analysis,J. Jpn Oil Chem. Soc. 34, 618–625.Google Scholar
  10. 10.
    Bagby, O.M., Smith, C.R. Jr., Mikolajczak, K.L., and Wolf, I.A. (1962)Thalictrum polycarpum Fatty Acids—A New Class of Fatty Acids from Vegetable Seed Oils,Biochemistry 1, 632–639.PubMedCrossRefGoogle Scholar
  11. 11.
    Wolff, R.M., and Bayard, C.C. (1995) Fatty Acid Composition of Some Pine Seed Oils,J. Am. Oil Chem. Soc. 72, 1043–1046.Google Scholar

Copyright information

© AOCS Press 1996

Authors and Affiliations

  • Tamao Saito
    • 1
  • Hiroshi Ochiai
    • 1
  1. 1.Division of Biological Sciences, Graduate School of ScienceHokkaido UniversitySapporoJapan

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