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Postnatal changes in activation of polyunsaturated fatty acid, acylation of lysophosphoglyceride, and phosphorylation of diacylglycerol in cortical gray matter of rat cerebrum

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Lipids

Abstract

Activation of polyunsaturated fatty acid, acylation of lysophosphoglyceride, and phosphorylation of diacylglycerol in postnatal developing rat cerebrum were studiedin vitro, using cortical gray matter homogenates. The rate of n-3 and n-6 fatty acid activation was maximal at ten days after birth and minimal at adulthood. Acylation of lysophosphoglyceride was most active at the neonatal stage and gradually decreased with age. In addition, the amount of phosphatidic acid formed from 1, 2-dioleoyl-glycerol was also maximum at the neonatal stage, and then gradually decreased. These results suggest that the specific activity of glycerolipid synthesis in cortical gray matter declines during postnatal development.

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Abbreviations

BSA:

bovine serum albumin

DG:

diacylglycerol

18∶1/18∶1 DG:

1, 2-dioleoyl-sn-glycerol

18∶0/20∶4 DG:

1-stearoyl-2-arachidonoyl-sn-glycerol

LPC:

lysophosphatidylcholine

LPEP:

lysophosphatidylethanolamine plasmalogen

LPG:

lysophosphoglyceride

PA:

phosphatidic acid

PC:

phosphatidylcholine

PE:

phosphatidylethanolamine

PUFA:

polyunsaturated fatty acid

References

  1. Innis, S.M. (1991). Essential Fatty Acids in Growth and Development,Prog. Lipid Res., 30, 39–103.

    Article  PubMed  CAS  Google Scholar 

  2. Korey, S.R., and Orchen, M. (1959) Plasmalogens of the Nervous System. I. Deposition in Developing Rat Brain and Incorporation of C14 Isotope from Acetate and Palmitate into the α, β-Unsaturated Ether Chain,Arch. Biochem. Biophys. 83, 381–389.

    Article  PubMed  CAS  Google Scholar 

  3. Naughton, J.M., and Trewhella, M.A. (1984) Positional Distribution of Acyl and Alk-1-Enyl groups in Grey and White Matter Ethanolamine and Choline Phosphoglycerides of a Marsupial, the Koala (Phascolarctos cinereus),J. Neurochem. 42, 685–691.

    Article  PubMed  CAS  Google Scholar 

  4. Sun, G.Y., and Horrocks, L.A. (1970). The Acyl and Alk-1-Enyl Groups of the Major Phosphoglycerides from Ox Brain Myelin and Mouse Brain Microsomal, Mitochondrial and Myelin Fractions,Lipids 5, 1006–1012.

    PubMed  CAS  Google Scholar 

  5. MacDonald, J.I.S., and Sprecher, H. (1991) Phospholipid Fatty Acid Remodeling in Mammalian Cells,Biochim. Biophys. Acta 1084, 105–121.

    PubMed  CAS  Google Scholar 

  6. Onuma Y., Masuzawa, Y., Ishima, Y., and Waku, K. (1984) Selective Incorporation of Docosahexaenoic Acid in Rat Brain,Biochim. Biophys. Acta 793, 80–85.

    PubMed  CAS  Google Scholar 

  7. Masuzawa, Y., Sugiura, T., Sprecher, H., and Waku, K. (1989) Selective Acyl Transfer in the Reacylation of Brain Glycerophospholipids. Comparison of Three Acylation Systems for 1-Alk-1′-Enylglycero-3-Phosphoethanolamine, 1-Acylglycero-3-Phosphoethanolamine and 1-Acylglycero-3-Phosphocholine in Rat Brain Microsomes,Biochim. Biophys. Acta 1005, 1–12.

    PubMed  CAS  Google Scholar 

  8. Portman, O.W., Illingworth, D.R., and Alexander, M. (1973) Lysolecithin and Sphingosinephosphorylcholine in the Metabolism of Brain Phospholipids of the Rhesus Monkey (Macaca mulatta): Effects of Development,J. Neurochem. 20, 1659–1667.

    Article  PubMed  CAS  Google Scholar 

  9. Natarajan, V., and Sastry, P.S. (1974) Enzymatic Acylation of 1-Alkyl-, 1-Alkenyl- and 1-Acyl Glycero-3-Phosphorylethanolamine in Developing Rat Brain,J. Neurochem. 23, 187–192.

    Article  PubMed  CAS  Google Scholar 

  10. Bazán, N.G., Aveldaño de Caldironi M.I., Giusto, N.M., and Rodríguez de Turco, E.B. (1981) Phosphatidic Acid in the Central Nervous System,Prog. Lipid Res. 20, 307–313.

    Article  PubMed  Google Scholar 

  11. Lapetina, E.G. and Hawthorne, J.N. (1971) The Diacylglyceride Kinase of Rat Cerebral Cortex,Biochem. J. 122, 171–179.

    PubMed  CAS  Google Scholar 

  12. Bell, R.M., and Coleman, R.A. (1980) Enzymes of Glycerolipid Synthesis in Eukaryotes,Annu. Rev. Biochem. 49, 459–487.

    Article  PubMed  CAS  Google Scholar 

  13. Kanazawa, A., Watanabe, Y., Shirota, Y., Sugimoto, T., and Fujimoto, K. (1995) Effect of Docosahexaenoic Acid Ethyl Ester Supplement for Lactating Rat on Lipid and Fatty Acid Compositions of Pup's Liver and Cerebral Cortex,J. Jpn. Soc. Nutr. Food Sci. 48, 173–180.

    CAS  Google Scholar 

  14. Reddy, T.S., and Bazan, N.G. (1984) Activation of Polyunsaturated Fatty Acids by Rat Tissuesin vitro, Int. J. Dev. Neuroscience 2, 447–450.

    Article  CAS  Google Scholar 

  15. Fisher S.K., and Rowe, C.E. (1980) The Acylation of Lysophosphatidylcholine by Subcellular Fractions of Guinea-pig Cerebral Cortex,Biochim. Biophys. Acta 618, 231–241.

    PubMed  CAS  Google Scholar 

  16. Bligh, E.G., and Dyer, W.J. (1959) A Rapid Method of Total Lipid Extraction and Purification,Can. J. Biochem. Physiol. 37, 911–917.

    PubMed  CAS  Google Scholar 

  17. Vitiello, F., and Zanetta, J.P. (1978) Thin-Layer Chromatography of Phospholipids,J. Chromatogr. 166, 637–640.

    Article  PubMed  CAS  Google Scholar 

  18. Kanoh, H., and Ohno, K. (1981) Partial Purification and Properties of Diacylglycerol Kinase from Rat Liver Cytosol,Arch, Biochem. Biophys. 209, 266–275.

    Article  CAS  Google Scholar 

  19. O'Brien, J.S., and Sampson, E.L. (1965) Fatty Acid and Fatty Aldehyde Composition of the Major Brain Lipids in Normal Human Gray Matter, White Matter, and Myelin,J. Lipid Res. 6, 545–551.

    PubMed  Google Scholar 

  20. Lemaitre, R.N., King, W.C., MacDonald, M.L., and Glomset, J.A. (1990) Distribution of Distinct Archidonoyl-specific and Non-Specific Isoenzymes of Diacylglycerol Kinase in Baboon (Papio cynocephalus) Tissues,Biochem. J. 266, 291–299.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aobaku, 981, Sendai, Japan

    Ayako Kanazawa, Yukari Watanabe & Kenshiro Fujimoto

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  1. Ayako Kanazawa
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  2. Yukari Watanabe
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  3. Kenshiro Fujimoto
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Kanazawa, A., Watanabe, Y. & Fujimoto, K. Postnatal changes in activation of polyunsaturated fatty acid, acylation of lysophosphoglyceride, and phosphorylation of diacylglycerol in cortical gray matter of rat cerebrum. Lipids 31, 437–440 (1996). https://doi.org/10.1007/BF02522932

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  • DOI: https://doi.org/10.1007/BF02522932

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