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Purification and characterization of aPenicillium sp. lipase which discriminates against diglycerides

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Lipids

Abstract

A lipase was isolated fromPenicillium sp. strain UZLM-4 and characterized. This lipase has a molecular weight of 27,344 (determined by mass spectrometry) and hydrolyzes triglycerides in preference to mono- and diglyceride substrates. Among various triglyceride substrates, tributyrin is hydrolyzed about four times faster than any other tested. The lipase has a preference for hydrolysis at the 1,3 positions of the lipids and shows a weak stereoselectivity for the S enantiomer. Unlike most other lipases, this lipase is stable and has a high activity at low surface pressures (5–10 mN/m).

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Abbreviations

HPLC:

high-performance liquid chromatography

PDMS:

poly(dimethylsiloxane)

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Authors and Affiliations

  1. Institute of Microbiology, Uzbek Academy of Sciences, Tashkent, Uzbekistan

    Komola Gulomova & Kahramon Davranov

  2. Biotechnology Research Institute, National Research Council, 6100 Royalmount Ave., H4P 2R2, Montreal, Quebec, Canada

    Edmund Ziomek, Joseph D. Schrag & Miroslaw Cygler

Authors
  1. Komola Gulomova
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  2. Edmund Ziomek
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  3. Joseph D. Schrag
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  4. Kahramon Davranov
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  5. Miroslaw Cygler
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Gulomova, K., Ziomek, E., Schrag, J.D. et al. Purification and characterization of aPenicillium sp. lipase which discriminates against diglycerides. Lipids 31, 379–384 (1996). https://doi.org/10.1007/BF02522923

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  • DOI: https://doi.org/10.1007/BF02522923

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