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Sterol specificity of theSaccharomyces cerevisiae ERG6 gene product expressed inEscherichia coli

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Lipids

Abstract

TheERG6 gene fromSaccharomyces cerevisiae has been functionally expressed inEscherichia coli, for the first time, yielding a protein that catalyzes the bisubstrate transfer reaction whereby the reactive methyl group from (S)-adenosyl-l-methionine is transferred stereoselectively to C-24 of the sterol side chain. The structural requirements of sterol in binding and catalysis were similar to the native protein fromS. cerevisiae. Inhibition of biomethylation was observed with fecosterol and ergosterol which suggests that ergosterol may function in wild-type yeast as a feedback regulator of sterol biosynthesis.

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Abbreviations

AdoMet:

(S)-adenosyl-l-methionine

HPLC:

high-performance liquid chromatography

ORF:

open reading frame

SMT:

(S)-adenosyl-l-methionine:Δ24(25)

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, Texas Tech University, P.O. Box 4260, 79409-1061, Lubbock, Texas

    Mylavarapu Venkatramesh, De-an Guo, James G. Harman & W. David Nes

Authors
  1. Mylavarapu Venkatramesh
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  2. De-an Guo
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  3. James G. Harman
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  4. W. David Nes
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Venkatramesh, M., Guo, Da., Harman, J.G. et al. Sterol specificity of theSaccharomyces cerevisiae ERG6 gene product expressed inEscherichia coli . Lipids 31, 373–377 (1996). https://doi.org/10.1007/BF02522922

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  • DOI: https://doi.org/10.1007/BF02522922

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