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Lipids

, Volume 31, Issue 7, pp 771–776 | Cite as

n-3 Polyunsaturated fatty acid-induced changes in the molecular species composition of diradylglycerol in murine peritoneal macrophages remain stable during incubationex vivo

  • Paola A. Marignani
  • Rolf J. Sebaldt
Communication

Abstract

Three- to four-week-old C57BL/6 mice were maintained for four weeks on diets in which the 10% lipid component was the ethyl esters of either corn oil or n-3 polyunsaturated fatty acids (n-3 PUFA). Incubation of macrophagesex vivo for 14 h, a normal incubation time used for macrophage studies, in the absence of fetal calf serum, did not diminish the extent of incorporation of 20∶5n-3 (eicosapentaenoic acid) and 22∶6n-3 (docosahexaenoic acid) moieties into membrane phospholipids and into diradylglycerol (DG) relative to that after a very abbreviated incubation time. We conclude that studies examining the effects of dietary n-3 PUFA on DG formation and related physiological effects in macrophages can be performed after a normalex vivo incubation of at least 14 h without experiencing a significant loss of incorporated n-3 PUFA.

Keywords

Molecular Species Diet Group Phorbol Myristate Acetate Fatty Acid Ethyl Ester Standard Duration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

AA

arachidonic acid

COEE

corn oil ethyl ester concentrate

DG

diradylglycerol

dfcs

heat-deactivated fetal calf serum

DHA

docosahexaenoic acid

EPA

eicosapentaenoic acid

PAF

1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine

PL

phospholipids

PMA

phorbol myristate acetate

PUFA

polyunsaturated fatty acids

n-3EE

n-3 fatty acid ethyl ester concentrate

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Copyright information

© AOCS Press 1996

Authors and Affiliations

  • Paola A. Marignani
    • 1
  • Rolf J. Sebaldt
    • 1
  1. 1.Department of Medicine, Faculty of Health SciencesMcMaster UniversityHamiltonCanada

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