n-3 Polyunsaturated fatty acid-induced changes in the molecular species composition of diradylglycerol in murine peritoneal macrophages remain stable during incubationex vivo
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Three- to four-week-old C57BL/6 mice were maintained for four weeks on diets in which the 10% lipid component was the ethyl esters of either corn oil or n-3 polyunsaturated fatty acids (n-3 PUFA). Incubation of macrophagesex vivo for 14 h, a normal incubation time used for macrophage studies, in the absence of fetal calf serum, did not diminish the extent of incorporation of 20∶5n-3 (eicosapentaenoic acid) and 22∶6n-3 (docosahexaenoic acid) moieties into membrane phospholipids and into diradylglycerol (DG) relative to that after a very abbreviated incubation time. We conclude that studies examining the effects of dietary n-3 PUFA on DG formation and related physiological effects in macrophages can be performed after a normalex vivo incubation of at least 14 h without experiencing a significant loss of incorporated n-3 PUFA.
KeywordsMolecular Species Diet Group Phorbol Myristate Acetate Fatty Acid Ethyl Ester Standard Duration
corn oil ethyl ester concentrate
heat-deactivated fetal calf serum
phorbol myristate acetate
polyunsaturated fatty acids
n-3 fatty acid ethyl ester concentrate
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- 8.Sebaldt, R.J. and Marignani, P.A. (1995) Diradylglycerol (DG) Formation Is Altered by n-3 Highly Unsaturated Fatty Acids with Marked Differences Between Eicosapentaenoic (EPA) and Docosahexaenoic (DHA) Acids: A Novel Mechanism of Action of n-3 Fatty Acids in Signal Transduction, inThe 3rd International Conference on Eicosanoids & Other Bioactive Lipids in Cancer, Inflammation & Radiation Injury, (Nigam, S., Honn, K.V., and Marnett, L.J., eds.) pp. 931–939, Kluwer Academic Publishers, Boston.Google Scholar