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Plasma concentrations of dihydro-vitamin K1 following dietary intake of a hydrogenated vitamin K1-rich vegetable oil

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Lipids

Abstract

Dihydro-vitamin K1 is a dietary form of vitamin K1 (phylloquinone) produced during the hydrogenation of vegetable oils. To determine if dihydro-vitamin K1 is present in plasma following dietary intake of a hydrogenated fat, eight healthy adults consumed each of two diets containing 30% of calories from fat, of which 20% was either soybean oil or a partially hydrogenated soybean oil-based stick margarine. Of the fats and oils analyzed, dihydro-vitamin K1 was only found in the hydrogenated products. The soybean oil diet contained 180 ±12 μg (mean±SD) of vitamin K1/day and nondetectable levels of dihydro-vitamin K1, whereas the stick margarine diet contained 199±7 μg of vitamin K1/day and 23±2 μg of dihydrovitamin K1/day. After consuming each diet for five weeks, plasma dihydro-vitamin K1 concentrations were higher (P=0.002) in all eight subjects when consuming the stick margarine diet (0.56 ±0.33 nmol/L) compared to the soybean oil diet (0.12±0.11 nmol/L). There was no significant change in plasma vitamin K1 concentrations when the two diets were compared. In conclusion, dihydro-vitamin K1 is detectable in plasma following dietary intake of a hydrogenated vitamin K1-rich vegetable oil.

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Abbreviations

FDA:

Food and Drug Administration

HPLC:

high-performance liquid chromatography

K1(25)' :

2-methyl-3-(3,7,11,15,19-pentamethyl-2-eicosenyl)-1,4-naphthalenedione

RDA:

recommended dietary allowance

SPE:

solid-phase extraction

TDS:

total diet study

References

  1. Booth, S.L., Sadowski, J.A., Weihrauch, J.L., and Ferland, G. (1993) Vitamin K1 (phylloquinone) Content of Foods: a Provisional Table,J. Food Comp. Anal. 6, 109–120.

    Article  CAS  Google Scholar 

  2. Booth, S.L., Sadowski, J.A., and Pennington, J.A.T. (1995) The Phylloquinone (vitamin K1) Content of Foods in the US-FDA Total Diet Study,J. Agric. Food Chem. 43, 1574–1579.

    Article  CAS  Google Scholar 

  3. Ferland, G., and Sadowski, J.A. (1992) The Vitamin K1 (phylloquinome) Content of Edible Oils: Effects of Heating and Light Exposure,J. Agric. Food Chem. 40, 1869–1873.

    Article  CAS  Google Scholar 

  4. Food and Nutrition Board (1989) Dietary Intake and Nutritional Status: Trends and Assessment, inDiet and Health, pp. 41–84. National Academy Press, Washington, D.C.

    Google Scholar 

  5. Applewhite, T.H. (1981) Nutritional Effects of Hydrogenated Soya Oil,J. Am. Oil Chem. Soc. 58, 260–269.

    CAS  Google Scholar 

  6. Booth, S.L., Davidson, K.W., and Sadowski, J.A. (1994) Evaluation of an HPLC Method for the Determination of Phylloquinone (vitamin K1) in Various Food Matrices,J. Agric. Food Chem. 42, 295–300.

    Article  CAS  Google Scholar 

  7. Davidson, K.W., Booth, S.L., Dolnikowski, G.G., and Sadowski, J.A. (1996) The Conversion of Phylloquinone to 2,3-Dihydro-Vitamin K1 During the Hydrogenation of Vegetable Oils,J. Agric. Food Chem. 44, 980–983.

    Article  CAS  Google Scholar 

  8. Suttie, J.W., Groossman, C.P, and Benton, M.E. (1992) Specificity of the Vitamin K and Glutamyl Binding Sites of the Liver Microsomal γ-Glutamyl Carboxylase,J. Nutr Sci Vit. Spec. No., 405–408.

  9. Fieser, L.F., Tishler, M., and Sampson, W.L. (1941) Vitamin K Activity and Structure,J. Biol. Chem. 137, 659–692.

    CAS  Google Scholar 

  10. Booth, S.L., Pennington, J.A.T., and Sadowski, J.A. (1996) Dihydro-Vitamin K1: Primary Food Sources and Estimated Dietary Intakes in the American Diet,Lipids 31, 715–720.

    PubMed  CAS  Google Scholar 

  11. Food and Nutrition Board (1989)Recommended Dietary Allowances, 10th edn., National Academy Press, Washington, D.C.

    Google Scholar 

  12. Langenberg, J.P., and Tjaden, U.R. (1984) Improved Method for the Determination of Vitamin K1 Epoxide in Human Plasma with Electrofluorometric Reaction Detection,J. Chromatogr. 289, 377–385.

    Article  PubMed  CAS  Google Scholar 

  13. Haroon, Y., Bacon, D.S., and Sadowski, J.A. (1986) Liquid-Chromatographic Determination of Vitamin K1 in Plasma, with Fluorometric Detection,Clin. Chem. 32, 1925–1929.

    PubMed  CAS  Google Scholar 

  14. LePage, G., and Roy, C. (1986) Direct Transesterification of All Classes of Lipids in a One-Step Reaction,J. Lipids Res. 27, 114–120.

    CAS  Google Scholar 

  15. Dam, H., Glavind, J., and Karrer, P. (1940) Die biologische Aktivität der natürlichen K-Vitamine und einiger verwandter Verbindungen,Helv. Chim. Acta 23, 224–233.

    Article  CAS  Google Scholar 

  16. Sadowski, J.A., Hood, S.J., Dallal, G.E., and Garry, P.J. (1989) Phylloquinone in Plasma from Elderly and Young Adults: Factors Influencing Its Concentration,Am. J. Clin. Nutr. 50, 100–108.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, 02111, Boston, Masachusets

    Sarah L. Booth, Kenneth W. Davidson, Alice H. Lichtenstein & James A. Sadowski

Authors
  1. Sarah L. Booth
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  2. Kenneth W. Davidson
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  3. Alice H. Lichtenstein
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  4. James A. Sadowski
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Booth, S.L., Davidson, K.W., Lichtenstein, A.H. et al. Plasma concentrations of dihydro-vitamin K1 following dietary intake of a hydrogenated vitamin K1-rich vegetable oil. Lipids 31, 709–713 (1996). https://doi.org/10.1007/BF02522886

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  • DOI: https://doi.org/10.1007/BF02522886

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