Effect of medium-chain triglycerides on calbindin-D9k expression in the intestine
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These studies determined the effect of the saturated fat source in infant formula on the expression of calbindin-D9k (CaBP-9k). Piglets were fed from birth to 8 d with milk or formula containing saturated fatty acids as medium-chain triglycerides (MCT), coconut oil, palm oil (Palm 1), or synthesized triglycerides with 16∶0 directed to thesn-2 position (Palm 2). Levels of intestinal CaBP-9k mRNA were significantly (P<0.01) higher in piglets fed formula with MCT than in piglets fed the other formula or milk; and higher in piglets fed the Palm-1 than in piglets fed Palm-2 formula. This is the first evidence that MCT alter piglet intestinal CaBP-9k mRNA.
KeywordsRepresentative Northern Blot Cesium Chloride Density Gradient Centrifugation Villus Axis Fatty Acid Accretion Cytosolic Calcium Binding Protein
- Palm-1 and Palm-2
16∶0 esterified predominantly to the triglyceride atsn-1,3 andsn-2, respectively
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