Abstract
During metamorphosis of bonefish (Albula sp.) larvae (leptocephali) all energy requirements are provided by breakdown of endogenous compounds, with lipid catabolism accounting for about 80% of total energy production. The principal objective of the present study was to characterize the lipid classes and fatty acids utilized. Analysis of whole-body lipid content indicated that larvae lost about half (3.6 mg) of their total lipid during the 10-d period. Percentages of neutral and polar lipid in early metamorphosing larvae were 64.2 and 35.8%, respectively; these values showed little change during metamorphosis, indicating that both lipid classes were catabolized. Triacylglycerols, the principal neutral lipid of all metamorphic stages, decreased by 1.8 mg. accounting for half of the decrease in total lipid. Levels of phosphatidylethanolamine, the principal polar lipid in early larvae, decreased by more than 50% during metamorphosis; levels of phosphatidylcholine, which was not detected in early larvae, increased. Fatty acids showing the largest net decreases, presumedly used as energy sources, were 16∶0 (30.4%), 14∶0 (13.8%), 16∶1n-7 (12.2%), 20∶5n-3 (7.7%), 18∶1n-9 (7.4%), and 18∶4n-3 (6.9%). Most of 22∶6n-3, the second most abundant fatty acid of early larvae, was conserved.
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Abbreviations
- FAME:
-
fatty acid methyl ester
- HPTLC:
-
high-performance thin-layer chromatography
- PC:
-
phosphatidylcholine
- PE:
-
phosphatidylethanolamine
- PUFA:
-
polyunsaturated fatty acids
- SL:
-
standard length
- TAG:
-
triacylglycerols
- TEM:
-
transmission electron microscopy
- TLC:
-
thin-layer chromatography
References
Pfeiler, E. (1984) Glycosaminoglycan Breakdown During Metamorphosis of Larval BonefishAlbula, Mar. Biol. Lett. 5, 241–249.
Pfeiler, E., and Luna, A. (1984) Changes in Biochemical Composition and Energy Utilization During Metamorphosis of Leptocephalous Larvae of the Bonefish (Albula),Env. Biol. Fish. 10, 243–251.
Pfeiler, E. (1984) Changes in Water and Salt Content During Metamorphosis of Larval Bonefish (Albula),Bull. Mar. Sci. 34, 177–184.
Pfeiler, E. (1984) Towards an Explanation of the Developmental Strategy in Leptocephalous Larvae of Marine Teleost Fishes,Env. Biol. Fish. 15, 3–13.
Rasquin, P. (1955) Observations on the Metamorphosis of the Bonefish,Albula vulpes (Linnaeus),J. Morphol. 97, 77–117.
Pfeiler, E. (1984) Inshore Migration, Seasonal Distribution and Sizes of Larval Bonefish,Albula, in the Gulf of California,Env. Biol. Fish. 10, 117–122.
Folch, J., Lees, M. and Sloane Stanley, G... (1957) A Simple Method for the Isolation and Purification of Total Lipides from Animal Tissues,J. Biol. Chem. 226, 497–509.
Dittmer, J.C., and Lester, R.L. (1964) A Simple, Specific Spray for the Detection of Phospholipids on Thin-Layer Chromatograms,J. Lipid Res. 5, 126–127.
Vaskovsky, V.E., and Kostetsky, E.Y. (1968) Modified Spray for the Detection of Phospholipids on Thin-Layer Chromatograms.J. Lipid Res. 9, 396.
Christie, W.W. (1982)Lipid Analysis, 2nd edn., Pergamon Press, New York.
Pfeiler, E., and Lindley, V. (1989) Chloride-Type Cells in the Skin of the Metamorphosing Bonefish (Albula sp.) Leptocephalus,J. Exp. Zool. 250, 11–16.
Kates, M. (1972) Techniques of Lipidology, inLaboratory Techniques in Biochemistry and Molecular Biology (Work, T.S., and Work, E., eds.) Vol. 3, Part II, pp. 267–610, American Elsevier, New York.
Otake, T., Nogami, K., and Maruyama, K. (1993) Dissolved and Particulate Organic Matter as Possible Food Sources for Eel Leptocephali,Mar. Ecol. Prog. Ser. 92, 27–34.
Donnelly, J., Torres, J.J., and Crabtree, R.E. (1995) Proximate Composition and Nucleic Acid Content of Premetamorphic Leptocephalus Larvae of the Congrid EelAriosoma balearicum, Mar. Biol., 123, 851–858.
Tocher, D.R., Fraser, A.J., Sargent, J.R., and Gamble, J.C. (1985) Fatty Acid Composition of Phospholipids and Neutral Lipids During Embryonic and Early Larval Development in Atlantic Herring (Clupea harengus L.),Lipids 20, 69–74.
Fraser, A.J., Gamble, J.C., and Sargent, J.R. (1988) Changes in Lipid Content, Lipid Class Composition and Fatty Acid Composition of Developing Eggs and Unfed Larvae of Cod (Gadus morhua),Mar. Biol. 99, 307–313.
Rainuzzo, J.R., Reitan, K.I., and Jørgensen, L. (1992) Comparative Study on the Fatty Acid and Lipid Composition of Four Marine Fish Larvae,Comp. Biochem. Physiol. 103B, 21–26.
Tocher, D.R., and Sargent, J.R. (1984) Analyses of Lipids and Fatty Acids in Ripe Roes of Some Northwest European Marine Fish,Lipids, 19, 492–499.
Fraser, A.J., Sargent, J.R., Gamble, J.C., and Seaton, D.D. (1989) Formation and Transfer of Fatty Acids in an Enclosed Marine Food Chain Comprising Phytoplankton, Zooplankton and Herring (Clupea harengus L.) Larvae,Mar. Chem. 27, 1–18.
Lie, O. (1993) Changes in Fatty Acid Composition of Neutral Lipids and Glycerophospholipids in Developing Cod Eggs, inPhysiological and Biochemical Aspects of Fish Development (Walther, B.T., and Fyhn, H.J., eds.) pp.330–337, University of Bergen, Norway.
Gerschenson, L.E., and Rotello, R.J. (1992) Apoptosis: A Different Type of Cell Death,FASEB J, 6, 2450–2455.
Vázquez, R., González, S., Rodríguez, A., and Mourente, G. (1994) Biochemical Composition and Fatty Acid Content of Fertilized Eggs, Yolk Sac Stage Larvae and First-Feeding Larvae of the Senegal Sole (Solea senegalensis Kaup),Aquaculture 119, 273–286.
Otwell, W.S., and Rickards, W.L. (1981) Cultured and Wild American Eels,Anguilla rostrata: Fat Content and Fatty Acid Composition,Aquaculture 26, 67–76.
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Padrón, D., Lindley, V.A. & Pfeiler, E. Changes in lipid composition during metamorphosis of bonefish (Albula sp.) leptocephali. Lipids 31, 513–519 (1996). https://doi.org/10.1007/BF02522645
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DOI: https://doi.org/10.1007/BF02522645