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Quantitative high-performance liquid chromatography analysis of plant phospholipids and glycolipids using light-scattering detection

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Lipids

An Erratum to this article was published on 01 May 1996

Abstract

Application of the evaporative light-scattering principle to quantitative high-performance liquid chromatography (HPLC) analyses of plant membrane lipids has received little study. Light-scattering detection response curves were generated for nine classes of plant membrane phospholipid and glycolipids. Quantitative results obtained by HPLC/light-scattering detection and conventional lipid analytical methods (thin-layer chromatography and lipid-P assay) were in close agreement, confirming the reliability of HPLC/evaporative light-scattering detection (ELSD) analyses. Only three of the nine plant lipid classes gave linear detector response functions above 10 μg injected lipid mass. This finding contradicts earlier precepts involving light-scattering detection of lipids. At a given mass, appreciable variation in ELSD signal intensity and detection limit was found to exist among the various plant membrane lipid classes. The variation in detector response among plant lipid classes is an important consideration in achieving accurate quantitative results in plant lipid analyses.

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Abbreviations

ASG:

acylated sterol glycoside

DGDG:

digalactosyldiacylglycerol

ELSD:

evaporative light-scattering detection

FID:

flame-ionization detection

HPLC:

high-performance liquid chromatography

LPC:

lysophosphatidylcholine

MGDG:

monogalactosyldiacylglycerol

PA:

phosphatidic acid

PC:

phosphatidylcholine

PE:

phosphatidylethanolamine

PI:

phosphatidylinositol

SG:

sterol glycoside

TLC:

thin-layer chromatography

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Authors and Affiliations

  1. Department of Agricultural Sciences, Technology, and Education, Louisiana Tech University, P.O. Box 10198, 71272, Ruston, Louisiana

    G. A. Picchioni

  2. Horticultural Crops Quality Laboratory, Beltsville Agricultural Research Center, U.S. Department of Agriculture, 20705, Beltsville, Maryland

    A. E. Watada & B. D. Whitaker

Authors
  1. G. A. Picchioni
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  2. A. E. Watada
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  3. B. D. Whitaker
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Additional information

An erratum to this article is available at http://dx.doi.org/10.1007/BF02522651.

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Picchioni, G.A., Watada, A.E. & Whitaker, B.D. Quantitative high-performance liquid chromatography analysis of plant phospholipids and glycolipids using light-scattering detection. Lipids 31, 217–221 (1996). https://doi.org/10.1007/BF02522623

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  • DOI: https://doi.org/10.1007/BF02522623

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