, Volume 31, Issue 1, pp 107–113 | Cite as

Biochemical effects of dietary linoleic/α-linolenic acid ratio in term infants

  • Craig L. Jensen
  • Huiming Chen
  • J. Kennard Fraley
  • Robert E. Anderson
  • William C. Heird
Symposium on Dietary Fat and Neural Development


Recent statements concerning linoleic (LA) and α-linolenic acid (LNA) intakes for infants include a desirable range of LA/LNA ratios. To evaluate several dietary LA/LNA ratios, the fatty acid patterns of plasma and erythrocyte phospholipid fractions, as well as plasma total lipid fractions, were determined shortly after birth and at 21, 60, and 120 d of age in term infants fed formula with 16% of fat as LA and either 0.4, 0.95, 1.7, or 3.2% as LNA (LA/LNA ratios of approximately 44, 18, 10, and 5). The content of all n-3 fatty acids in both plasma fractions was higher at all times in infants who received the highest LNA intake; however, the docosahexaenoic acid (DHA) content was only half that shortly after birth or reported in breast-fed infants of comparable ages. The LA content of plasma lipids of all groups was higher at all times than shortly after birth but did not differ among groups. The arachidonic acid (AA) content was higher in infants who received the lowest LNA intake, but only half that at birth or reported in breast-fed infants. In contrast, the DHA content of the erythrocyte phospholipid fraction did not differ among groups until 120 d of age when it was higher in those who received the highest LNA intake and the AA content of this fraction did not differ among groups at any time. These data demonstrate that dietary LA/LNA ratios between 5 and 44 do not result in plasma or erythrocyte lipid levels of DHA or plasma lipid levels of AA similar to those at birth or reported by others in breast-fed infants. However, the data indicate that the LA/LNA ratio of the formula is an important determinant of the amounts of DHA and AA required to achieve plasma and erythrocyte levels of these fatty acids similar to those of breast-fed infants.


Infant Formula Term Infant Stearin Phospholipid Fraction Plasma Phospholipid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



arachidonic acid


docosahexaenoic acid


eicosapentaenoic acid


linoleic acid


α-linolenic acid


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  1. 1.
    Clandinin, M.T., Chappel, J.E., Leong, S., Heim, T., Swyer, P.R., and Chance, S.W. (1980) Extrauterine Fatty Acid Accretion in Infant Brain: Implications for Fatty Acid Requirements,Early Hum. Dev. 4, 131–138.PubMedCrossRefGoogle Scholar
  2. 2.
    Sastry, P.S. (1985) Lipids of Nervous Tissue: Composition and Metabolism,Prog. Lipid. Res. 24, 69–176.PubMedCrossRefGoogle Scholar
  3. 3.
    Fliesler, S.J., and Anderson, R.E. (1983) Chemistry and Metabolism of Lipids in the Vertebrate Retina,Prog. Lipid Res. 22, 79–131.PubMedCrossRefGoogle Scholar
  4. 4.
    Innis, S.M. (1991) Essential Fatty Acids in Growth and Development,Prog. Lipid. Res. 30, 39–103.PubMedCrossRefGoogle Scholar
  5. 5.
    Carlson, S.E., Rhodes, P.G., and Ferguson, M.G. (1986) Docosahexaenoic Acid Status of Preterm Infants at Birth and Following Feeding with Human Milk or Formula,Am. J. Clin. Nutr. 44, 798–804.PubMedGoogle Scholar
  6. 6.
    Innis, S.M., Foote, K.D., Mackinnon, M.J., and King, P.L. (1990) Plasma and Red Blood Cell Fatty Acids of Low-Birth-Weight Infants Fed Their Mother’s Expressed Breast Milk or Preterm Infant Formula,Am. J. Clin. Nutr. 51, 994–1000.PubMedGoogle Scholar
  7. 7.
    Clark, K.J.U., Makrides, M., Neumann, M.A., and Gibson, R.A., (1992) Determination of the Optimal Ratio of Linoleic Acid to α-Linolenic Acid in Infant Formulas,J. Pediatr. 120, S151–158.CrossRefGoogle Scholar
  8. 8.
    Ponder, D.L., Innis, S.M., Benson, J.D., and Siegman, J.S. (1992) Docosahexaenoic Acid Status of Term Infants Fed Breast Milk or Infant Formula Containing Soy Oil or Corn Oil,Pediatr. Res. 32, 683–688.PubMedGoogle Scholar
  9. 9.
    Innis, S.M., Nelson, C.M., Rioux, M.F., and King, D.J. (1994) Development of Visual Acuity in Relation to Plasma and Erythrocyte ω-6 and ω-3 Fatty Acids in Healthy Term Gestational Infants,Am. J. Clin. Nutr. 60, 347–352.PubMedGoogle Scholar
  10. 10.
    Uauy, R.D., Birch, D.G., Birch, E.E., Tyson, J.E., and Hoffman, D.R. (1990) Effect of Dietary ω3 Fatty Acids on Retinal Function of Very-Low-Birth-Weight Neonates,Pediatr. Res. 28, 485–492.PubMedGoogle Scholar
  11. 11.
    Makrides, M., Simmer, K., Goggin, M., and Gibson, R.A. (1993) Erythrocyte Docosahexaenoic Acid Correlates with the Visual Response of Healthy Term Infants,Pediatr. Res. 33, 425–427.PubMedCrossRefGoogle Scholar
  12. 12.
    Makrides, M., Neumann, M.A., Byard, R.W., Simmer, K., and Gibson, R.A. (1994) Fatty Acid Composition of Brain, Retina, and Erythrocytes in Breast- and Formula-Fed Infant,Am. J. Clin. Nutr. 60, 189–194.PubMedGoogle Scholar
  13. 13.
    Martinez, M. (1992) Tissue Levels of Polyunsaturated Fatty Acids During Early Human Development,J. Pediatr. 120, S129-S138.PubMedCrossRefGoogle Scholar
  14. 14.
    Farquaharson, J., Jamieson, E.C., Abbasi, K.A., Patrick, W.J., Logan, R.W., and Cockburn, F. (1995) Effect of Diet on the Fatty Acid Composition of the Major Phospholipids of Infant Cerebral Cortex,Arch. Dis. Child. 72, 198–203.CrossRefGoogle Scholar
  15. 15.
    Carnielli, V.P., Wattimena, D.J.L., Luijendijk, I.H.T., Boerlage, A., Degenhart, H.J., and Sauer, P.J.J. (1994) Chain Elongation and Desaturation of Linoleic (LL) and Linolenic (LN) Acid in the VLBW Infant: Effect of Dietary Long Chain Polyunsaturated Fatty Acids (LCP),Pediatr. Res. 35, 309A.Google Scholar
  16. 16.
    Demmelmair, H., Rinke, U., Behrendt, E., Sauerwald, T., and Koletzko, B. (1995) Estimation of Arachidonic Acid Synthesis in Fullterm Neonates Using Natural Variation of13C-Abundance,J. Pediatr. Gastroenterol. Nutr. 21, 31–36.PubMedCrossRefGoogle Scholar
  17. 17.
    Sauerwald, T., Jensen, C.L., Chen, H.M., Anderson, R.E., Heird, W.C., and Hachey, D.L. (1995) Effect of Dietary 18:3ω3 Intake and Postnatal Age on the Kinetics of Elongation and Desaturation of 18:2ω6 and 18:3ω3,Pediatr. Res. 37, 319AGoogle Scholar
  18. 18.
    ESPGAN Committee on Nutrition (1991) Committee Report: Comment on the Content and Composition of Lipids in Infant Formulas,Acta. Paediatr. Scand. 80, 887–896.Google Scholar
  19. 19.
    ISSFAL Board Statement (1994) Recommendations for the Essential Fatty Acid Requirements for Infant Formulas,ISSFAL Newsletter 1, 4–5.Google Scholar
  20. 20.
    The British Nutrition Foundation (1992) Unsaturated Fatty Acids. Nutritional and Physiological Significance, inThe Report of the British Nutrition Foundation’s Task Force, pp 63–67, 152–163, T.J. Press (Padstow) Ltd., Padstow, Cornwall.Google Scholar
  21. 21.
    Food and Agriculture Organization/World Health Organization (1994)Fats and Oils in Human Nutrition, pp. 6–7, 49–55, FAO/WHO, Rome.Google Scholar
  22. 22.
    Koletzko, B. (1995) Long-Chain Polyunsaturated Fatty Acids in Infant Formulae in Europe.ISSFAL Newsletter 2 3–5.Google Scholar
  23. 23.
    Van Aerde, J.E., and Clandinin, M.T. (1993) Controversy in Fatty Acid Balance,Can. J. Physiol. Pharmacol. 71, 707–712.PubMedGoogle Scholar
  24. 24.
    Hamill, P.V.V., Drizd, T.A., Johnson, C.L., Reed, R.B., Roche, A.F., and Moore, W.M. (1979) Physical Growth: National Center for Health Statistics Percentiles,Am. J. Clin. Nutr. 32, 607–629.PubMedGoogle Scholar
  25. 25.
    Bligh, E.G., and Dyer, W.J. (1959) A Rapid Method of Total Lipid Extraction and Purification,Can. J. Biochem. Physiol. 37, 911–917.PubMedGoogle Scholar
  26. 26.
    Morrison, W.R., and Smith, L.M. (1964) Preparation of Fatty Acid Methyl Esters and Dimethylacetals from Lipids with Boron Fluoride-Methanol,J. Lipid Res. 5, 600–608.PubMedGoogle Scholar
  27. 27.
    Wiegand, R.D., and Anderson, R.E. (1982) Determination of Molecular Species of Rod Outer Segment Phospholipids,Meth. in Enzymol. 81, 297–304.CrossRefGoogle Scholar
  28. 28.
    Anderson, R.E., Maude, M.B., Acland, G.M., and Aguirre, G.D. (1994) Plasma Lipid Changes in Pred-Affected and Normal Miniature Poodles Given Oral Supplements of Linseed Oil. Indications for the Involvement of n-3 Fatty Acids in Inherited Retinal Degenerations,Exp. Eye Res. 58, 129–137.PubMedCrossRefGoogle Scholar
  29. 29.
    Mantzioris, E., James, M.J., Gibson, R.A., and Cleland, L.G. (1995) Differences Exist in the Relationships Between Dietary Linoleic and Alpha-Linolenic Acids and Their Respective Long-Chain Metabolites,Am. J. Clin. Nutr. 61, 320–324.PubMedGoogle Scholar
  30. 30.
    Voss, A., Reinhart, M., Sankarappa, S., and Sprecher, H. (1991) The Metabolism of 7,10,13,16,19-Docosapentenoic Acid to 4,7,10,13,16,19-Docosahexaenoic Acid in Rat Liver Is Independent of a 4-Desaturase,J. Biol. Chem. 266, 19995–20000.PubMedGoogle Scholar
  31. 31.
    Sprecher, H. (1992) Interconversions Between 20-and 22-Carbon n-3 and n-6 Fatty Acidsvia 4-Desaturase Independent Pathways, inEssential Fatty Acids and Eicosanoids (Sinclair, A., and Gibson, R., eds.) pp. 18–22, American Oil Chemists’ Society, Champaign.Google Scholar
  32. 32.
    Hachey, D.L., Sauerwald, T., Jensen, C.L., Anderson, R.E., and Heird, W.C. (1995) An Alternative Pathway for Elongation and Desaturation of 18:3ω3 in Term Infants,Pediatr. Res. 37, 122A.Google Scholar
  33. 33.
    Sauerwald, T.U., Jensen, C.L., Chen, H.M., Anderson, R.E., Heird, W.C., and Hachey, D.L. (1996) Dretary α-Linolenic Acid Intake Suppresses Incorporation of Arachidonic Acid into Plasma Phospholipid,Lipids, in press.Google Scholar
  34. 34.
    Mohammed, B.S., Sankarappa, S., Geiger, M., and Sprecher, H. (1995) Ree valuation of the Pathway for the Metabolism of 7,10,13,16-Docosatetraenoic Acid to 4,7,10,13,16-Docosapentaenoic Acid in Rat Liver,Arch. Biochem. Biophysics. 317, 179–184.CrossRefGoogle Scholar
  35. 35.
    Sprecher, H., and Baykousheva, S. (1994) The Role Played by Beta-Oxidation in Unsaturated Fatty Acid Biosynthesis,World Rev. Nutr. Dietetics 75, 26–29.Google Scholar
  36. 36.
    Koletzko, B., Thiel, I., and Abiodun, P.O. (1992) The Fatty Acid Composition of Human Milk in Europe and Africa,J. Pediatr. 120, S62-S70.PubMedCrossRefGoogle Scholar
  37. 37.
    Henderson, R.A., Jensen, R.G., Lammi-Keefe, C.J., Ferris, A.M., and Dardick, K.R. (1992) Effect of Fish Oil on the Fatty Acid Composition of Human Milk and Maternal and Infant Erythrocytes,Lipids 27, 863–869.PubMedGoogle Scholar
  38. 38.
    Hrboticky, N., MacKinnon, M.J., and Innis, S.M. (1990) Effect of Vegetable Oil Formula Rich in Linoleic Acid on Tissue Fatty Acid Accretion in the Brain, Liver, Plasma and Erythrocytes of Infant Piglets,Am. J. Clin. Nutr. 51, 173–182.PubMedGoogle Scholar
  39. 39.
    Carlson, S.E., Carver, J.D., and House, S.G. (1986) High Fat Diets Varying in Ratios of Polyunsaturated to Saturated Fatty Acid and Linoleic to Linolenic Acid: A Comparison of Rat Neural and Red Cell Membrane Phospholipids,J. Nutr. 116, 718–725.PubMedGoogle Scholar
  40. 40.
    Martinez, M., Ballabriga, A., and Gil-Gibernau, J.J. (1988) Lipids of the Developing Retina: I. Total Fatty Acids, Plasmalogens, and Fatty Acid Composition of Ethanolamine and Choline Phosphoglycerides,J. Neuroscience. Res. 20, 484–490.CrossRefGoogle Scholar
  41. 41.
    Auestad, N., Montalto, M.B., Wheeler, R.E., Fitzgerald, K.R., Hall, R.T., Neuringer, M., Connor, W.E., Hartmann, E.E., and Taylor, J.A. (1995) Visual Acuity, Erythrocyte Fatty Acids and Growth in Term Infants Fed Formulas With and Without Long Chain Polyunsaturated Fatty Acids (LCP),Pediatr. Res. 37, 302A.Google Scholar

Copyright information

© AOCS Press 1996

Authors and Affiliations

  • Craig L. Jensen
    • 2
  • Huiming Chen
    • 1
  • J. Kennard Fraley
    • 2
  • Robert E. Anderson
    • 1
  • William C. Heird
    • 2
  1. 1.Departments of Ophthalmology and BiochemistryBaylor College of MedicineHouston
  2. 2.Department of PediatricsUSDA/ARS Children’sNutrition Research Center, Baylor College of MedicineHouston

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