Cytotechnology

, Volume 9, Issue 1–3, pp 125–130 | Cite as

Physiology of myeloma cells grown in glucose-limited chemostat cultures

  • P. R. Levering
  • J. A. M. van Heijst
  • C. M. G. Sünnen
Article

Abstract

A recombinant myeloma NS1-derived clone was grown in chemostat cultures in Dulbecco's MEM/Ham's F12 (1∶1) medium containing various concentrations of glucose, at a dilution rate of 0.028 h−1. Serum-supplemented cultures were virtually glucose-limited at a large range of glucose feed concentrations (0.7–5 mM). True glucose-limited cultures, however, were only established at low glucose supply levels to 1.3 mM at a maximum. In cultures obtained at higher glucose concentrations methionine was shown to be the growth-limiting compound. The pattern derived for serum-free chemostat cultures was similar, except that growth yields on glucose were much lower. Glucose was shown to be the growth-limiting substrate in cultures fed with media containing less than 4.5 mM glucose. Upon supplying glucose at higher concentrations such cultures presumably run into methionine and/or tryptophan limitation.

Key words

amino acids chemostat glucose-limitation methionine myeloma NS1 

Abbreviations

Ylac,glc

yield of lactate on glucose

Yx,glc

yield of biomass on glucose

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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • P. R. Levering
    • 1
  • J. A. M. van Heijst
    • 1
  • C. M. G. Sünnen
    • 1
  1. 1.Department of Biotechnology and Biochemistry, Scientific Development GroupOrganon International BVOssThe Netherlands

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