, Volume 9, Issue 1–3, pp 69–75 | Cite as

Novel cell lines derived from transgenic mice expressing recombinant human proteins

Transgenic hepatoma-derived cell lines
  • Frédéric Perraud
  • Wilfried Dalemans
  • Dalila Ali-Hadji
  • Andrea Pavirani


We have used transgenic mouse technology to establish immortalized hepatoma cell lines stably secreting heterologous proteins, such as human α1-antitrypsin and human factor IX. Hepatocyte-specific regulatory DNA sequences were used to target both the expression of anonc gene and the gene coding for the human protein to the liver of transgenic mice which eventually developed hepatocellular carcinomas. Tumour cells were subsequently established as permanent cell lines, which maintained a differentiated phenotype under specific culture conditions, being capable of producing biologically active and correctly processed human α1-antitrypsin and factor IX. Moreover, a preliminary analysis has shown that certain cell lines express elevated total cytochrome P450 activity. These cells could therefore represent a useful alternative to the use of animals or primary cultures in drug safety testing.

Key words

transgenic mice trans-immortalization hepatocyte α1-antitrypsin factor IX onc gene 


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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Frédéric Perraud
    • 1
  • Wilfried Dalemans
    • 1
  • Dalila Ali-Hadji
    • 1
  • Andrea Pavirani
    • 1
  1. 1.Department of Molecular and Cellular BiologyTransgene S.A.Strasbourg CedexFrance

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