Abstract
Phosphorus-32 and chlorine-36 radioactivity was measured directly in commercially available tissue solubilizers using a liquid scintillation counter. Various wavelength-shifting compounds: β-naphthol, 4-methylumbelliferone, 7-amino-1,3-naphtalenedisulfonic acid, 2-hydroxy-3,6-naphthalenedisulfonic acid, anthranilic acid and salicylic acid were investigated to assess their suitability for the improvement of counting efficiency. Salicylic acid was selected which is fairly stable in alkaline solutions of tissue solubilizers and remarkably improves counting efficiency up to 90% for both nuclides. 0.1 g of soft tissues or blood can be solubilized with 1 cm3 of tissue solubilizer containing 2 g/dm3 of salicylic acid directly in 6 cm3 scintillation minivials. The sample channels ratio method for colour quench correction was found to be satisfactory.
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Bem, E.M., Bem, H. & Reimschüssel, W. Direct determination of32P or36Cl in tissue solubilizer solutions by Cherenkov-scintillation counting. J. Radioanal. Chem. 79, 69–76 (1983). https://doi.org/10.1007/BF02518852
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DOI: https://doi.org/10.1007/BF02518852