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Determination of the optical purity of L-selenomethionine-75Se

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Abstract

The method developed for the determination of the optical purity of L-selenomethionine-75Se is based on the conversion of the mixture of L-and D-amino acids into the diastereoisomeric derivatives by reaction with (−)-camphorsulphonyl chloride. The diastereoisomeric derivatives are separated by means of thin layer chromatography. From the ratio of the radioactivities of the two spots on the chromatogram, the optical purity is calculated, and given in % D. For a sample of L-methionine-14C the following value was found: 0.53±0.05 (S.D. of mean, n=7). For a sample of DL-methionine-14C the percentage of D found, was: 48.5±0.5 (S. D. of mean, n=7). Of 30 different samples of L-selenomethionine-75Se the percentages of D-isomer were determined in duplicate. From the 30 differences between duplicates the S. D. of a single measurement is calculated to be 0.2.

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References

  1. KYUNG J. CHO, BRUSE D. DOREST, J. Nucl. Med., 15 (1974) 1171.

    CAS  Google Scholar 

  2. M.A. WINSTON, P. GUTH, J.S. ENDOW, W.H. BLAHD, J. Nucl. Med., 15 (1974) 662.

    CAS  Google Scholar 

  3. A.G. MATTAR, Y. LAOR, J. Nucl. Med., 15 (1974) 707.

    CAS  Google Scholar 

  4. P.PH.L. OTTO, Dutch Patent, Appl. No. 69.08609.

  5. J.P. GREENSTEIN, M. WINITZ, Chemistry of the Amino Acids, Wiley, New York, 1961, p. 715.

    Google Scholar 

  6. M.J. HARDY, Anal. Biochem., 52 (1974) 529.

    Article  Google Scholar 

  7. L. PICHAT, J.P. BEAUCOURT, J. Labelled Compd., 10 (1974) 103.

    Article  CAS  Google Scholar 

  8. D.F. HOLMES, R. ADAMS, J. Am. Chem. Soc., 56 (1934) 2093.

    Article  CAS  Google Scholar 

  9. A.W. INGERSOLL, J. Am. Chem. Soc., 47 (1925) 1168.

    Article  CAS  Google Scholar 

  10. S. SMILES, Th.P. HILDITCH, J. Chem. Soc., 91 (1907) 519.

    CAS  Google Scholar 

  11. A. LIGHT, E.L. SMITH, in The Proteins, Vol. I, 2nd ed. H. NEURATH (ed.) Academic Press, New York, 1963, p. 38.

    Google Scholar 

  12. W.J. POPE, A.W. HARVEY, J. Chem. Soc., 79 (1901) 74.

    Google Scholar 

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Authors and Affiliations

  1. Centraal Laboratorium TNO, Delft, (The Netherlands)

    P. Ph. H. L. Otto & G. F. Van Veen-Van Staalduinen

Authors
  1. P. Ph. H. L. Otto
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  2. G. F. Van Veen-Van Staalduinen
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Otto, P.P.H.L., Van Veen-Van Staalduinen, G.F. Determination of the optical purity of L-selenomethionine-75Se. J. Radioanal. Chem. 35, 37–44 (1977). https://doi.org/10.1007/BF02518210

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  • DOI: https://doi.org/10.1007/BF02518210

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