Application of microscale liquid chromatographic technique to the anion exchange separation of halide ions

Abstract

The microscale liquid chromatograph used in the present work is as follows: injection volume of sample 0.2–0.9 μl; column 0.5 mm i.d x 12–59 mm; connecting tube between column and detection cell 0.25 or 0.5 mm i.d. x 20 mm; detection cell 0.25 or 0.5 mm i.d. x 8 mm. The high performance anion exchange resin, TSK LS-222, was loadd in the column, and applied to the separation of halide ions. Three halides, Cl⁻, Br⁻ and I⁻ have been separated with a column of 59 mm length within 19 min, using only 152 μl of 1.5N sodium nitrate-acetone (1:1) mixture as carrier solution. When the element of interest is only iodine, it could be separated from the other halides with a column of 12 mm length within 4.5 min. Some fundamental studies for HETP and capacity factor are presented.