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Properties of the apamin-sensitive component of Ca2+-dependent K+ current in smooth muscle cells of the guinea pigtaenia coli

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Abstract

With the help of a standard voltage-clamp technique, we investigated transmembrane ion currents in isolated smooth muscle cells of the guinea pigtaenia coli. In Ca2+-dependent K+ current, we identified and studied the properties of an apamin-sensitive voltage-independent component carried through the channels of low conductance (in many publications called small conductance,I SK(Ca)). This component did not show the temporal inactivation;I SK(Ca) was insensitive to the action of 4 mM tetraethylammonium, but was completely blocked by 500 nM of apamin. It was shown thatI SK(Ca) is very sensitive to changes in the intracellular Ca2+ concentration ([Ca2+] i ): a decrease in [Ca2+] i up to 50 nM resulted in the almost complete blockade of the current. The entry of Ca ions into a cell from the external solution through the voltage-operated Ca2+ channels of L-type was not an obligatory condition for activation ofI SK(Ca). The current-voltage relationship forI SK(Ca) had a maximum within the voltage range of +20 to +50 mV.

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Authors and Affiliations

  1. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine

    A. V. Povstyan, A. V. Zima, M. I. Harhun & M. F. Shuba

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  1. A. V. Povstyan
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  2. A. V. Zima
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  3. M. I. Harhun
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  4. M. F. Shuba
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Neirofiziologiya/Neurophysiology, Vol. 32, No. 2, pp. 87–94, March–April, 2000.

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Povstyan, A.V., Zima, A.V., Harhun, M.I. et al. Properties of the apamin-sensitive component of Ca2+-dependent K+ current in smooth muscle cells of the guinea pigtaenia coli . Neurophysiology 32, 63–69 (2000). https://doi.org/10.1007/BF02515170

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  • DOI: https://doi.org/10.1007/BF02515170

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