, Volume 32, Issue 1, pp 1–7 | Cite as

Properties of the charibdotoxin-sensitive component of Ca2+-dependent K+ current in smooth muscle cells of the guinea pigTaenia Coli

  • A. V. Povtyan
  • A. V. Zima
  • M. I. Harhun
  • M. F. Shuba


Using the voltage-clamp technique, we investigated transmembrane ion currents in isolated smooth muscle cells of the guinea pigtaenia coli. In our study, we identified and studied a charibdotoxin-sensitive component of Ca2+-dependent K+ current carried through the channels of high conductance (in most publications called “big conductance,”I BK(Ca)). This component was completely blocked by 100 nM charibdotoxin and by tetraethylammonium in concentrations as low as 1 mM.I BK(Ca) demonstrated fast kinetics of inactivation, which nearly coincided with that of Ca2+ current. In addition to the dependence on Ca2+ concentration, this current also showed voltage-dependent properties: with a rise in the level of depolarization its amplitude increased. In many cells, depolarizing shifts in the membrane potential evoke spontaneous outward currents. Such currents probably represent the secondary effect of cyclic Ca2+ release from the caffeine-sensitive intracellular stores that result in short-term activation of charibdotoxin-sensitive Ca2+-dependent K+ channels.


smooth muscle cells Ca2+-dependent K+ channels charibdotoxin spontaneous outward currents 


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Copyright information

© Kluwer Academic/Plenum Publishers 2000

Authors and Affiliations

  • A. V. Povtyan
    • 1
  • A. V. Zima
    • 1
  • M. I. Harhun
    • 1
  • M. F. Shuba
    • 1
  1. 1.Bogomolets Institute of PhysiologyNational Academy of Sciences of UkraineKyivUkraine

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