Effect of long-term depolarization on the morphological characteristics of rat pheochromocytoma cells
Changes in the morphological characteristics of rat PC12 pheochromocytoma cells caused by incubation in the medium with a high KCl content were studied. On the third day of culturing in the modified medium containing 20 or 30 mM KCl, the proliferating activity of the cells was enhanced, and the total length of the dendrites and their number increased. Culturing of the cells in the medium containing 40 mM KCl resulted in significant suppression of the cell vital functions: retraction of the processes and blocking of proliferation were observed. Thus, chronic depolarization caused by a high potassium content in the extracellular medium activates proliferation of PC12 cells, which is probably related to the change in the level of free cytosolic calcium.
KeywordsPC12 Cell Nerve Growth Factor High Potassium Content Nerve Growth Factor Withdrawal PCI2 Cell
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