, Volume 31, Issue 1, pp 59–61 | Cite as

Effects of nitric oxide and hypoxia on low-and high-voltage activated calcium currents in murine DRG neurons

  • N. A. Solov’yova
  • P. G. Kostyuk
Poster Communications


The effects of a nitric oxide (NO)-containing aqueous solution (authentic NO) and hypoxia on low-and high-voltage activated calcium currents (I Ca,lva andI Ca,hva , respectively; in the latter transient and sustained portions were differentiated) were studied in enzymatically dispersed medium-sized neuronal somata from the murine dorsal root ganglia (DRG). Authentic NO (10 μM) was found to decrease the mean peak amplitude ofI Ca,lva , from 3.5±0.3 to 1.2±0.2 nA (n=11,p<0.001), as well as the amplitudes of transient and sustainedI Ca,hva components from 4.5±0.1 to 2.7±0.2 nA and form 2.8±0.2 to 1.7±0.2 nA (n=11;P<0.001), respectively. This NO-induced suppression was reversible and was removed by 1-min-long washout. At the same time, medium-sized DRG neurons demonstrated relatively low sensitivity to hypoxia (PO2=20–25 mm Hg): decreases of both types ofI Ca under hypoxic condition were not statistically significant (n=11;p>0.05). The data strongly suggest that NO is capable of reversibly suppressing both types of calcium channels in murine DRG neurons and of modulating in this way their excitability. It seems likely that this ability is based on a direct effect of NO on the corresponding channels and not on NO participation in the induction of hypoxic effects. Yet, a hypothesis that NO is a messenger of hypoxic damage to neural cells still should be suggested.


Nitric Oxide Dorsal Root Ganglion Tyrode Solution Nitric Oxide Concentration Sympathetic Preganglionic Neuron 
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  1. 1.
    S. Y. Wu, S. L. Dun, U. Forstermann, and N. J. Dun, “Nitric oxide and excitatory postsynaptic currents in immature rat sympathetic preganglionic neuronsin vitro,”Neuroscience,79, 237–245 (1997).PubMedCrossRefGoogle Scholar
  2. 2.
    C. Gazevieille, A. Muller, F. Meynier, and C. Bonne, “Super-oxide and nitric oxide cooperation in hypoxia/reoxygenation-induced neuron injury,”Free Radical Biol. Med.,14, 389–395 (1993).CrossRefGoogle Scholar
  3. 3.
    F. Groenendaal, O. P. Mishra, J. E. McGowan, et al., “Function of cell membranes in cerebral cortical tissue of newborn piglets after hypoxia and inhibition of nitric oxide synthase,”Pediatr. Res.,42, 174–179 (1997).PubMedGoogle Scholar
  4. 4.
    S. A. Fedulova, P. G. Kostyuk, and N. S. Veselovsk, “Two types of calcium channels in the somatic membrane of newborn rat dorsal root ganglion neurons,”J. Physiol.,359, 431–446 (1985).PubMedGoogle Scholar
  5. 5.
    R. S. Scroggs and A. P. Fox, “Calcium current variation between acutely isolated adult rat dorsal root ganglion neurons of different size,”J. Physiol.,445, 639–658 (1992).PubMedGoogle Scholar
  6. 6.
    O. P. Hamill, A. Marty, E. Neher, et al., “Improved patch clamp techniques for high-resolution current recording from cell and cell-free membrane patches,”Pflügers Arch.,391, 85–100 (1981).PubMedCrossRefGoogle Scholar
  7. 7.
    H. G. Bohlen, “Mechanism of increased vessel wall nitric oxide concentrations during intestinal absorbtion,”Am. J. Physiol.,275, No. 2, 542–550 (1998).Google Scholar
  8. 8.
    G. F. Clough, A. R. Bennett, and M. K. Church, “Measurement of nitric oxide concentration in human skinin vivo using dermal microdialysis,”Exp. Physiol.,83, No. 3, 431–434 (1998).PubMedGoogle Scholar
  9. 9.
    V. L. Dawson, T. M. Dawson, E. D. London, et al., “Nitric oxide mediated glutamate neurotoxicity in primary cortical culture,”Proc. Natl. Acad. Sci. USA,8, 6368–6371 (1991).CrossRefGoogle Scholar
  10. 10.
    V. M. Bolotina, S. Najibi, J. J. Palacino, et al., “Nitric oxide directly activates calcium dependent potassium channels in vascular smooth muscle,”Nature,368, 850–853 (1994).PubMedCrossRefGoogle Scholar

Copyright information

© Kluwer Academic/Plenum Publishers 1999

Authors and Affiliations

  • N. A. Solov’yova
    • 1
  • P. G. Kostyuk
    • 1
  1. 1.Bogomolets Institute of PhysiologyNational Academy of Sciences of UkraineKyivUkraine

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