Abstract
Optical tweezers are used mechanically to manipulate the plasma membrane of polymophonuclear leukocytes attached to the bottom of a glass manipulation chamber. The laser trapping beam is dragged across the membrane of cells in calcium-containing and calcium-depleted extracellular medium. This treatment causes a significant rise in the intracellular calcium concentration compared with controls, in cells in calcium-containing medium (239.8±49.0% against 75.4±16.4%, respectively), but not in cells in calcium-depeleted medium (69.1±9.6% against 83.4±18.5%, respectively), indicating that the calcium rise is caused by an influx of calcium from the environment. The rise in calcium concentration is blocked (23.5±7.1% against 17.1±4.1%, respectively) by the addition of lansoprazole, indicating that the influx is not due to unspecific membrane damage caused by the mechanical manipulation of the cell. It can therefore be concluded that mechanical manipulation of the neutrophil membrane, in the piconewton force range exerted by the optical tweezer, does not damage the plasma membrane but stimulates a mechanically inducible, membrane channel-mediated influx of extracellular calcium.
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References
Ashkin, A., andDziedzic, J. M. (1987): ‘Optical trapping and manipulation of viruses and bacteria’,Science,235, pp. 1517–1520
Ashkin, A., Dziedzic, J. M., andYamane, T. (1987): ‘Optical trapping and manipulation of single cells using infrared laser beams’,Nature,330, pp. 769–771
Ashkin, A., Schutze, K., Dziedzic, J. M., Euteneuer, U., andSchliwa, M. (1990): ‘Force generation of organelle transport measuredin vivo by an infrared laser trap’,Nature,348, pp. 346–348
Aybay, C., Imir, T., andOkur, H. (1995): ‘The effect of omeprazole on human natural killer cell activity’,Gen. Pharmac.,26, pp. 1413–1418
Block, S. M., Blair, D. F., andBerg, H. C. (1989): ‘Compliance of bacterial flagella measured with optical tweezers’,Nature,338, pp. 514–518
Block S. M., Goldstein, L. S. B., andSchnapp, B. J. (1990): ‘Bead movement by single kinesin molecules studied with optical tweezers’,Nature,348, pp. 348–352
Edidin, M., Kuo, S. C., andSheetz, M. P. (1991): ‘Lateral movements of membrane glycoproteins restricted by dynamic cytoplasmic barriers’,Science,254, pp. 1379–1382
Johansson, B., andMagnusson, K.-E. (1990): ‘Oxygen metabolites induced by phorbol myristate acetate increase lateral diffusion of wheat germ agglutinin-labelled glycoconjugates in human polymorphonuclear leukocytes’,Inflammation,14, pp. 613–644
Konig, K., Svaasand, L., Liu, Y., Sonek, G., Patrizio, P., Tadir, Y., Berns, M. W., andTromberg, B. J. (1996): ‘Determination of motility forces of human spermatozoa using an 800 nm optical trap’,Cell. Mol. Biol.,42, pp. 501–509
Krause, K. H. (1991): ‘Ca2+-storage organelles’,FEBS Lett.,285, pp. 225–229
Kusumi, A., andSako, Y. (1996): ‘Cell surface organisation by the membrane skeleton’,Curr. Opin. Cell Biol.,8, pp. 566–573
Liu, Y., Sonek, G. J., Berns, M. W., andTromberg, B. J. (1996): ‘Physiological monitoring of optically trapped cells: Assessing the effects of confinement by 1064 nm laser tweezers, using microfluorometry’,Biophys. J.,71, pp. 2158–2167.
Nanda, A., Brumell, J. H., Nordstrom, T., Kjeldsen, L., Sengelov, H., Borregaard, N., Rotstein, O. D., andGrinstein, S. (1996): ‘Activation of proton pumping in human neutrophils occurs by exocytosis of vesicles bearing vacuolar-type H+-ATPases’,J. Biol. Chem.,27, pp. 15963–15970
Nishizaka, T., Miyata, H., Yoshikawa, H., Ishiwata, S., andKinosita, K. (1995): ‘Unbinding force of a single motor molecule of muscle measured using optical tweezers’,Nature,377, pp. 251–254
Sandborg, R. R., andSmolen, J. E. (1988): ‘Early biochemical events in leukocyte activation’,Lab. Invest.,59, pp. 300–320
Scaringi, L., Cornacchione, P., Fettuciari, K., Rosati, E., Rossi, R., Marconi, P., andCapodicasa, E. (1996): ‘Activity inhibition of cytolytic lymphocytes by omeprazole’,Scand. J. Immun.,44, pp. 204–214
Speake, T., Douglas, I. J., andBrown, P. D. (1998): ‘The role of calcium in the volume regulation of rat lacrimal acinar cells’,J. Membr. Biol.,164, pp. 283–291
Stoddard, J. S., Steinbach, J. H., andSimchowitz, L. (1993): ‘Whole cell Cl-currents in human neutrophils induced by cell swelling’,Am. J. Physiol.,265, pp. 156–165
Suzuki, M., Nakamura, M., Mori, M., andMiura, S. (1995): ‘Lansoprazole inhibits oxygen-derived free radical production from neutrophils activated by Helicoabcter pylori’,J. Clin. Gastroenterol.,20, pp. 93–96
Uchida, M., Sato-Maeda, M., andTashiro H. (1995): ‘Micromanipulation. Whole-cell manipulation by optical trapping’,Curr. Biol.,5, pp. 380–382
Wandall, J. H. (1992): ‘Effects of omeprazole on neutrophil chemotaxis, super oxide production, degranulation, and translocation of cytochrome b245’,Gut,33, pp. 617–621
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Holm, Å., Sundqvist, T., Öberg, Å. et al. Mechanical manipulation of polymorphonuclear leukocyte plasma membranes with optical tweezers causes influx of extracellular calcium through membrane channels. Med. Biol. Eng. Comput. 37, 410–412 (1999). https://doi.org/10.1007/BF02513321
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DOI: https://doi.org/10.1007/BF02513321