Those mutants were studied whose defects resulted in the morphological changes of inflorescences inArabidopsis thaliana. We characterized newly isolatedcorymbosa mutants andacaulis5 mutants.
Thecorymbosa1-1 mutation was caused by the defects in the elongation of pedicels and the previously identifiederecta mutation belonged to this class. Thecorymbosa2-1 mutation was caused mainly by the increase of the number of the floral buds in the inflorescence.
The expression of theERECTA gene whose defect resulted to the corymbose inflorescence was analyzed. TheERECTA gene was expressed in subsets of cells in both the peripheral zone and central zone and was thought to have important role for the development of inflorescences.
The phenotypes of theacaulis5 mutation was pronounced just after the transition from the vegetative to reproductive growth phase. We found that the expressions of the genes for EXGT-A1 and γ-TIP were drastically reduced in theacaulis5 mutants.
Acaulis5 mutation Arabidopsis thalianaCorymbosa mutation ERECTA gene Inflorescence development
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Konieczny, A. andAusubel, F.M. 1993. A procedure for mappingArabidopsis mutations using co-dominant ecotype-specific PCR-based markers. Plant J.4: 403–410.PubMedCrossRefGoogle Scholar
Koornneef, M. andVan der Veen, J.H. 1980. Induction and analysis of GA sensitive mutants inArabidopsis thaliana. Theor. Appl. Genet.58: 257–263.CrossRefGoogle Scholar
Kopczak, S.D., Haas, N.A., Hussen, R.T., Silflow, C.D. andSnustad, D.P. 1992. The small genome ofArabidopsis contains at least six expressed α-tubulin genes. Plant Cell4: 539–547.PubMedCrossRefGoogle Scholar
Ludevid, D., Hofte, H., Himelblau, E. andChrispeels, M.J. 1992. The expression pattern of the tonoplast intrinsic protein γ-TIP inArabidopsis thaliana is correlated with cell enlargement. Plant Physiol.100: 1633–1639.PubMedCrossRefGoogle Scholar
Nishitani, K. andTominaga, R. 1992. Endo-xyloglucan transferase, a novel class of glycosyltransferase that catalyzes transfer of a segment of xyloglucan molecule to another xyloglucan molecule. J. Biol. Chem.267: 21058–21064.PubMedGoogle Scholar
Okazawa, K., Sato, Y., Nakagawa, T., Asada, K., Kato, I., Tomita, E. andNishitani, K. 1993. Molecular cloning and cDNA sequencing of endoxyloglucan transferase, a novel class of glycosyltransferase that mediates molecular grafting between matrix polysaccharides in plant cell walls. J. Biol. Chem.268: 25364–25368.PubMedGoogle Scholar
Phillips, A.L., Ward, D.A., Uknes, S., Appleford, N.E.J., Lange, T., Huttly, A.K., Gaskin, P., Graebe, J.E. andHedden, P. 1995. Isolation and expression of three gibberellin 20-oxidase cDNA clones fromArabidopsis. Plant Physiol.108: 1049–1057.PubMedCrossRefGoogle Scholar
Smyth, D.R., Bowman, J.L. andMeyerowitz, E.M. 1990. Early flower development inArabidopsis. Plant Cell2: 755–767.PubMedCrossRefGoogle Scholar
Snustad, D.P., Haas, N.A., Kopczak, S.D. andSilflow, C.D. 1992. The small genome ofArabidopsis contains at least nine expressed β-tubulin genes. Plant Cell4: 549–556.PubMedCrossRefGoogle Scholar
Torii, K.U., Mitsukawa, N., Oosumi, T., Matsuura, Y., Yokoyama, R., Whittier, R.F. andKomeda, Y. 1996. The ArabidopsisERECTA gene encodes a putative receptor protein kinase with extracellular leucine-rich repeats. Plant Cell8: 735–746.PubMedCrossRefGoogle Scholar
Xu, Y.-L., Li, L., Wu, K., Peeters, A.J.M., Gage, D.A. andZeevaart, J.A.D. 1995. The GA5 locus ofArabidopsis thaliana encodes a multifunctional gibberellin 20-oxidase: molecular cloning and functional expression. Proc. Natl. Acad. Sci. USA92: 6640–6644.PubMedCrossRefGoogle Scholar
Yamaguchi-Shinozaki, K., Koizumi, M., Urao, S. andShinozaki, K. 1992. Molecular cloning and characterization of 9 cDNAs for genes that are responsive to desiccation inArabidopsis thaliana: sequence analysis of one cDNA clone that encodes a putative transmembrane channel protein. Plant Cell Physiol.33: 217–224.Google Scholar