Abstract
Morphological and structural studies carried out inXanthoria parietina reveal some fungal mechanisms to regulate both growth and development of the phycobiont as well as the number of photobiont cells present in the holobiont. This regulation is performed by phenolic acids and glycosyl-enzymes.
An ultrastructural analysis using the polysaccharide detection technique PATAg shows that plasmolysis of cells occur when freshly isolated phycobionts are incubated with two arginase lectins (ABP, algal binding protein and SA, secreted arginase), with development of large cytoplasmic vesicles filled with amorphous polysaccharides that are exocyted to the periplasmic space. Finally, membranes of organelles and plasma membrane are altered and the cell wall is broken.
The results presented here provide evidence of a possible fungus-to-algal action as deduced from the hemiparasitic symbiosis theory.
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Abbreviations
- SA:
-
secreted arginase
- ABP:
-
algal binding protein
- PATAg:
-
periodique acide-thiocarbohydrazide-argent
- PBS:
-
phosphate saline buffer
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Molina, M.d.C., Bajon, C., Sauvanet, A. et al. Detection of polysaccharides and ultrastructural modification of the photobiont cell wall produced by two arginase isolectins fromXanthoria parietina . J. Plant Res. 111, 191–197 (1998). https://doi.org/10.1007/BF02512169
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DOI: https://doi.org/10.1007/BF02512169