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Persistence of complexed acidic phospholipids in rapidly mineralizing tissues is due to affinity for mineral and resistance to hydrolytic attack:In Vitro data

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Abstract

Acidic phospholipids, complexed with calcium and inorganic phosphate, are components of extracellular matrix vesicles. Both the complexed acidic phospholipids and matrix vesicles have previously been shown to serve as hydroxyapatite (HA) nucleators in solution and when implanted in a muscle pouch. The present study supplies evidence that complexed acidic phospholipids can persist in mineralizing tissues both because of their affinity for HA and because of their resistance to hydrolysis by phospholipase A2. Calcium-phosphatidylserine-phosphate complex (CPLX-PS) synthesized with14C-labeled phosphatidylserine (PS) was used to measure CPLX-PS affinity for HA using a Langmuir adsorption isotherm model. The affinity was shown to be higher and more specific than that of PS itself (K=8.66 ml/μmol; N, the number of binding sites =20.4 μmol/m2 as compared with previously reported values for PS of K=3.33 ml/μmol, and N=4.87 μmol/m2). Incorporated into synthetic liposomes and incubated in a calcium phosphate solution in which mineralization is induced by an ionophore, CPLX-PS showed behavior distinct from free PS. As previously reported, PS in these liposomes totally blocked HA formation. On the other hand, CPLX-PS in similar concentrations had a varied response, having no effect, slightly inhibiting, or actually promoting HA formation. CPLX-PS was also shown to be a poorer substrate for phospholipase A2 than PS, with Km=4.63 mM for CPLX-PS and Km=0.27 mM for PS; and Vmax=0.029 ml/minute for CPLX-PS and Vmax=0.066 ml/minute for PS. These data explain how complexed acidic phospholipids may persist in the growth plate and facilitate initial mineral deposition.

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Authors and Affiliations

  1. The Hospital for Special Surgery affiliated with The Cornell University Medical College New York, 10021, New York

    A. L. Boskey, W. Ullrich, L. Spevak & H. Gilder

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  1. A. L. Boskey
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  2. W. Ullrich
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  3. L. Spevak
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  4. H. Gilder
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Boskey, A.L., Ullrich, W., Spevak, L. et al. Persistence of complexed acidic phospholipids in rapidly mineralizing tissues is due to affinity for mineral and resistance to hydrolytic attack:In Vitro data. Calcif Tissue Int 58, 45–51 (1996). https://doi.org/10.1007/BF02509545

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  • DOI: https://doi.org/10.1007/BF02509545

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