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Molecular cytogenetic characterisation of the terminal heterochromatic segment of the B-chromosome of rye (Secale cereale)

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Abstract

The terminal heterochromatic segments of the long arms of 20 rye B-chromosomes were isolated by means of laser microdissection technology. Also the remaining portions of the long arms, along with the short arms of the same chromosomes were isolated. Each sample was used for degenerate oligonucleotide primer-polymerase chain reaction (DOP-PCR) amplification reactions. The resulting products were used as probes for chromosome in situ hybridisation experiments, and in Southern hybridisation to digests of 0B and +B DNA. Competition hybridisation of these probes with 0B DNA allowed the detection of B-specific sequences. The terminal heterochromatin of the rye B-chromosome contains both B-specific sequences and sequences also present on the A-chromosomes of rye. The B-specific D1100 family is the major repeat species located in the terminal heterochromatin. Primers designed to the cloned sequence (E1100) were used to search for related low copy sequences in 0B DNA. The sequences of the PCR products revealed no similarities to that of the clone E1100 except for the primer sequences. The possible origin of this sequence is discussed in the context of models for the evolution of the rye B-chromosome.

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Author notes

  1. Andreas Houben

    Present address: Department of Genetics, The University of Adelaide, 5005, South Australia, Australia

Authors and Affiliations

  1. Institut fur Pflanzengenetik und Kulturpflanzenforschung, D-06466, Gatersleben, Germany

    Andreas Houben, Ralf G. Kynast, Ute Heim & Hanno Hermann

  2. Genetics Group, Institute of Biological Sciences, University of Wales, Aberystwyth, SY 23 3DD, Dyfed, Wales, UK

    R. Neil Jones & John W. Forster

Authors
  1. Andreas Houben
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  2. Ralf G. Kynast
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  3. Ute Heim
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  4. Hanno Hermann
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  5. R. Neil Jones
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  6. John W. Forster
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Additional information

EMBL and Genbank accession numbers: Z54196 (E1199): Z54278 (B1)

Edited by: R. Appels

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Houben, A., Kynast, R.G., Heim, U. et al. Molecular cytogenetic characterisation of the terminal heterochromatic segment of the B-chromosome of rye (Secale cereale). Chromosoma 105, 97–103 (1996). https://doi.org/10.1007/BF02509519

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  • DOI: https://doi.org/10.1007/BF02509519

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