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Early salt-stress effects on expression of genes for aquaporin homologues in the halophyte sea aster (Aster tripolium L.)

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Abstract

Six partial PCR fragments that encode aquaporin homologues in the halophyte sea asterAster tripolium were cloned using genomic DNA and cDNA as templates. The deduced amino acid sequences were highly similar to those of other major intrinsic proteins (MIPs) in plants. The expression patterns of sea astermip genes in leaves and suspension-cultured cells during a 24-hr NaCL stress period were studied with PCR samip (sea aster major intrinsic protein) fragments. Three samip (A-C) fragments were obtained by PCR with genomic DNA as templates, and 3 samip (D-F) fragments were obtained by RT-PCR. Among the 6 genes, expression ofSamip A, B, andC was significantly inducible by salt stress. The level ofSamip A, B andC mRNAs was dependent on NaCL concentration in both whole plants and cultured cells. However, no significant accumulation ofSamip transcripts was observed in salt-adapted cells. These observations suggest thatSamip A, B andC genes play an important role in the early salt-adaptable stage in sea asters.

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Abbreviations

MIP:

major intrinsic protein

P-5-C:

Δ1-pyrroline-5-carboxylase

RT-PCR:

reverse transcription PCR

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Authors and Affiliations

  1. Division of Environmental Science, The Graduate School of Science & Technology, Kobe University, 1-1 Rokko., 657, Kobe, Japan

    Yuichi Uno, Noboru Inagaki & Susumu Maekawa

  2. Biological Resources Division, Japan International Research Center for Agricultural Science (JIRCAS), Ministry of Agriculture, Forestry and Fisheries, Ohwashi 1-2, 305, Tsukuba, Ibaraki, Japan

    Yuichi Uno, Takeshi Urao & Kazuko Yamaguchi-Shinozaki

  3. Laboratory of Plant Molecular Biology, The Institute of Physical and Chemical Research (RIKEN), Tsukuba, Life Science Center, 3-1-1, Koyadai, 305, Tsukuba, Ibaraki, Japan

    Yuichi Uno & Kazuo Shinozaki

  4. Faculty of Agriculture, Kobe University, 1-1, Rokko, 657, Kobe, Japan

    Yuichi Uno & Michio Kanechi

Authors
  1. Yuichi Uno
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  2. Takeshi Urao
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  3. Kazuko Yamaguchi-Shinozaki
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  4. Michio Kanechi
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  5. Noboru Inagaki
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  6. Susumu Maekawa
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  7. Kazuo Shinozaki
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Corresponding author

Correspondence to Kazuko Yamaguchi-Shinozaki.

Additional information

The nucleotide sequences of samip A, B, C, D, E and F cDNAs reported in this paper have been submitted to EMBL, Gen-Bank and DDBJ with accession numbers AB012267, AB012268, AB012269, AB012270, AB012271 and AB012272, respectively.

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Uno, Y., Urao, T., Yamaguchi-Shinozaki, K. et al. Early salt-stress effects on expression of genes for aquaporin homologues in the halophyte sea aster (Aster tripolium L.). J. Plant Res. 111, 411–419 (1998). https://doi.org/10.1007/BF02507806

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  • DOI: https://doi.org/10.1007/BF02507806

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