Journal of Plant Research

, Volume 111, Issue 4, pp 557–564 | Cite as

Variation in therbcL sequence ofStegnogramma pozoi subsp.mollissima (Thelypteridaceae) in Japan

  • Yoko Yatabe
  • Masayuki Takamiya
  • Noriaki Murakami
Original Articles


Stegnogramma pozoi (Lagasca) K. Iwats. subsp.mollissima (Fischer ex Kunze) K. Iwats. is one of the most common ferns in Japan with a very broad geographical distribution and habitat ranges. We examined the intrasubspecific variation of therbcL gene, and found three types in the subspecies (Atami-1, Atami-2, and Tateyama types). The Atami-1 and 2 types shared three apomorphic nucleotides and their geographical distribution is intermingled. Thus, we considered them to have intraspecific variation. In contrast, the Tateyama type had 4–5 different nucleotides than the Atami types, and showed a more northern distribution. Leaf morphology of the Tateyama type is distinct from that of the Atami types, though the leaf shape in Tateyama type varies considerably and in some cases overlaps with other types. Ferns have fewer morphological features that are systematically significant than flowering plants because they lack complex reproductive organ. It is reasonable to expect that species recognition of ferns based only on leaf morphology can be fine tuned by molecular features. Variation in therbcL sequences might be useful as an indication of significant biological units.

Key words

Intraspecific variation Plasticity rbcL Stegnogramma pozoi subsp.mollissima Thelypteridaceae 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Dewit, T.J., Sih, A. andWilson, A.S. 1998. Costs and limits of phenotypic plasticity. Trends Ecol. Evol.13: 77–81.CrossRefGoogle Scholar
  2. Doyle, J.J. andDoyle, J.L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf material. Phytochem. Bull.19: 11–15.Google Scholar
  3. Felsentein, J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution39: 783–791.CrossRefGoogle Scholar
  4. Haufler, C.H. andRanker, T.A. 1995.RbcL sequences provide phylogenetic insights among sister species of the fern genusPolypodium. Amer. Fern. J.85: 361–374.CrossRefGoogle Scholar
  5. Hauk, W.D. 1995. A molecular assessment of relationships among cryptic species ofBotrychium subgenusBotrychium (Ophioglossaceae). Amer. Fern. J.85: 375–394.CrossRefGoogle Scholar
  6. Hasebe M., Oumori, T., Nakazawa, M., Sano, T., Kato, M. andIwatsuki, K. 1994.RbcL gene sequences provide new evidence for the evolutionary lineages of leptosprangiate ferns. Proc. Natl. Acad. Sci. USA91: 5730–5734.PubMedCrossRefGoogle Scholar
  7. Hirabayashi, H. 1969. Chromosome numbers in several species of the Aspidiaceae. J. Jap. Bot.44: 113–119.Google Scholar
  8. Hirabayashi, H. 1970. Chromosome numbers in several species of the Aspidiaceae (2). J. Jap. Bot.45: 45–52.Google Scholar
  9. Iwatsuki, K. 1963. Taxonomic studies of Pteridophyta VII. Acta Phytotax. Geobot.19: 112–126.Google Scholar
  10. Iwatsuki, K. 1995. Flora of Japan. Kodansha, Tokyo.Google Scholar
  11. Kurita, S. 1960. Chromosome, numbers of some Japanese ferns. J. Jap. Bot.35: 269–272 (in Japanese with English summary).Google Scholar
  12. Kurizono, S. 1987. Cytological studies on some taxa in thelypteroid ferns, Aspidiaceae. Kromosomo II46: 1513–1520.Google Scholar
  13. Maddison, W.P. andMaddison, D.R. 1992. MacClade, Version 3.0. Sinauer Associates. Sunderland.Google Scholar
  14. Manton I. andSledge, W.A. 1954. Observation on the cytology and taxonomy of the pteridophyte flora of Ceylon. Philos. Trans. Roy. Soc. London B238: 127–185.Google Scholar
  15. Masuyama, S. 1992. Clinal variation of frond morphology and its adaptive implication in the fernCeratopteris thalictroides in Japan. Pl. Species Biol.7: 87–96.CrossRefGoogle Scholar
  16. Masuyama, S. andWatano, Y. 1994. Hybrid sterility between two isozymic types of the fernCeratopteris thalictroides in Japan. J. Plant Res.107: 269–274.CrossRefGoogle Scholar
  17. Mitui K. 1968. Chromosomes and speciation in fern. Sci. Rep. Tokyo Kyoiku Daigaku, Sec. B13: 285–333.Google Scholar
  18. Murakami, N. 1995. Systematics and evolutionary biology of the fern genusHymenasplenium (Aspleniaceae). J. Plant Res.108: 257–268.CrossRefGoogle Scholar
  19. Phipps, C.J., Taylor, T.N., Taylor, E.L., Cuneo, N.R., Boucher, L.D. andYao, X. 1998.Osmuda (Osmundaceae) from the Triassic of Antarctica: an example of evolutionary stasis. Amer. J. Bot.85: 888–895.CrossRefGoogle Scholar
  20. Serizawa, S. 1983. On JapaneseStegnogramma pozoi subsp.mollissima. Bull. Jap. Pterid. Soc.56: 3–5 (in Japanese).Google Scholar
  21. Swofford, D. 1993. Phylogenetic Analysis Using Parsimony, Version 3.1. Sinauer Associates, Sunderland.Google Scholar
  22. Tagawa, M. 1959. Colored lllustrations of the Japanese Pteridophyta. Hoikusha, Osaka (in Japanese).Google Scholar
  23. Templeton, A.R. 1989. The meaning of species and speciation: A genetic perspective.In D. Otto and J. A. Endler, eds., Speciation and Its Consequences, Sinauer Associates, Sunderland, pp. 3–27.Google Scholar
  24. Watano, Y. andMasuyama, S. 1994. Genetic differentiation in populations of the polymorphic fernCeratopteris thalictroides in Japan. J. Plant Res.107: 139–146.CrossRefGoogle Scholar

Copyright information

© The Botanical Society of Japan 1998

Authors and Affiliations

  • Yoko Yatabe
    • 1
  • Masayuki Takamiya
    • 2
  • Noriaki Murakami
    • 1
  1. 1.Department of Botany, Graduate School of ScienceKyoto UniversityKyotoJapan
  2. 2.Department of Environmental Science, Faculty of ScienceKumamoto UniversityKumamotoJapan

Personalised recommendations