Abstract
Genes for 19-kDa and 42-kDa subunits of protoplast-release-inducing protein (PR-IP) were cloned from theClosterium peracerosum-strigosum-littorale complex mt+ cells. Genomic sequences and transcriptional initiation sites for both genes were determined. Sequence data revealed that typical TATA boxes are located immediately upstream of both transcriptional initiation sites. Several similar sequences were observed at nearly the same location in the promoters of both genes. Based on these data, we propose some possible hypotheses regulating the expression of the PR-IP subunit genes.
Similar content being viewed by others
References
Banuett, F., 1995. Genetics ofUstilago maydis, a fungal pathogen that induces tumors in maize. Annu. Rev. Genetics29: 179–208.
Bölker, M. andKahmann, R. 1993. Sexual pheromones and mating responses in fungi. Plant Cell5: 1461–1469.
Bölker, M., Urban, M. andKahmann, R. 1992. The a mating-type locus ofUstilago maydis specifies cell signaling components. Cell68: 441–450.
Edwards, J.B.D.M., Delort, J. andMallet, J. 1991. Oligodeoxyribonucleotide ligation to single-stranded cDNAs: a new tool for doing 5′ ends of mRNAs and for constructing cDNA libraries byin vitro amplification. Nucleic Acids Res.19: 5227–5232.
Ferris, P.J. andGoodenough, U.W. 1997. Mating type inChlamydomonas is specified bymid, the minus-dominance gene. Genetics146: 859–869.
Hamada, J., Yoshizawa-Katoh, T. andTsunewaki, K. 1982. Genetics study on mating type genes by a new type of tetrad analysis inClosterium ehrenbergii. Bot. Mag. Tokyo95: 101–108.
Ichimura, T. 1971. Sexual cell division and conjugation-papilla formation in sexual reproduction ofClosterium strigosum.In K. Nishizawa, ed., Proc. of the 7th Int. Seaweed Symp., University of Tokyo Press, Tokyo, pp. 208–214.
Kasai, F. andIchimura, T. 1990. A sex determining mechanism in theClosterium ehrenbergii (Chlorophyta) species complex. J. Phycol.26: 195–201.
Nojiri, T., Fujii, T. andSekimoto, H. 1995. Purification and characterization of a novel sex pheromone that induces the release of another sex pheromone during sexual reproduction of the heterothallicClosterium peracerosum-strigosum-littorale complex. Plant Cell Physiol.36: 79–84.
Sambrook, J., Fritsch, E.F. andManiatis, T. 1989. Molecular Cloning, A laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
Sekimoto, H., Satoh, S. andFujii, T. 1990. Biochemical and physiological properties of a protein inducing protoplast release during conjugation in theClosterium peracerosum-strigosum-littorale complex. Planta182: 348–354.
Sekimoto, H., Sone, Y. andFujii, T. 1994a. Regulation of expression of the genes for a sex pheromone by an inducer of the sex pheromone in theClosterium peracerosum-strigosum-littorale complex. Planta193: 137–144.
Sekimoto, H., Sone, Y. andFujii, T. 1994b. cDNA cloning of a 42-Kilodalton subunit of protoplast-release-inducing protein fromClosterium. Plant Physiol.104: 1095–1096.
Sekimoto, H., Sone, Y. andFujii, T. 1994c. A cDNA encoding a 19-Kilodalton subunit of protoplast-release-inducing protein fromClosterium. Plant Physiol.105: 447.
Shure, M., Wessler, S. andFederoff, N. 1983. Molecular identification of theWaxy locus in maize. Cell35: 225–233.
Wendland, J., Vaillancourt, L.J., Hegner, J., Lengeler, K.B., Laddison, K.J., Specht, C.A., Raper, C.A andKothe, E. 1995. The mating-type locusBal ofSchizophyllum commune contains a pheromone receptor gene and putative pheromone genes. EMBO J.14: 5271–5278.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Endo, B., Fujii, T., Kamiya, Y. et al. Analysis of genomic sequences encoding a sex pheromone from theClosterium peracerosum-strigosum-littorale complex. J. Plant Res. 110, 463–467 (1997). https://doi.org/10.1007/BF02506807
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02506807