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Chromatographia

, Volume 45, Issue 1, pp 355–363 | Cite as

Synthesis and characterization of a chemically-bonded cholesteric stationary phase for high-performance liquid chromatogrphy

  • C. Delaurent
  • V. Tomao
  • A. M. Siouffi
Originals

Summary

A bonded, cholesteric stationary phase for high performance liquid chromatography was prepared by reacting the cholesteryl chloroformate with the terminal amino group of a bonded aminopropyl silica from Merck. Infrared Fourier transform spectrometry; carbon-13 cross polarization magic angle spinning NMR and differential scanning calorimetry confirmed the success of the bonding reaction. Carbon elemental analysis indicated a high loading of the cholesteric moiety on the surface and the argon adsorption method was used to measure the specific surface of this new bonded stationary phase. Van't Hoff plots of both cholesteric phase and RP-18 confirm the different behaviour. Examples of selectivities are given.

Key Words

Column liquid chromatography Cholesterol-bonded phase Spectroscopic and thermoanalytical characterization Chromatographic behaviour 

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References

  1. [1]
    Z. Witkiewicz, Z. Suprynowicz, J. Wojcik, R. Dabrowski, J. Chromatogr.152, 328 (1978).Google Scholar
  2. [2]
    F. Jassen, Anal. Chem.51, 2163 (1979).CrossRefGoogle Scholar
  3. [3]
    Z. Witkiewicz, J. Chromatogr.251, 311 (1982).CrossRefGoogle Scholar
  4. [4]
    Z. Witkiewicz, J. Mazur, LC-GC8, 224 (1990).Google Scholar
  5. [5]
    J. J. Pesek, T. Cash, Chromatographia27, 559 (1987).CrossRefGoogle Scholar
  6. [6]
    J. J. Pesek, M. A. Vidensek, M. Miller, J. Chromatogr.556, 373 (1991).CrossRefGoogle Scholar
  7. [7]
    J. E. Sandoval, J. J. Pesek, Anal. Chem.63, 2634 (1991).CrossRefGoogle Scholar
  8. [8]
    C.-H. Chu, E. Jonsson, M. Auvinen, J. J. Pesek, J. E. Sandoval, Anal. Chem.65, 808 (1993).CrossRefGoogle Scholar
  9. [9]
    J. J. Pesek, V. H. Tatang, Chromatographia39, 649 (1994).CrossRefGoogle Scholar
  10. [10]
    J. J. Pesek, M. T. Matyska, E. J. Williamsen, R. Tam, Chromatographia41, 30 (1995).Google Scholar
  11. [11]
    A. Thienpont, G. Felix, Chromatographia37, 505 (1993).CrossRefGoogle Scholar
  12. [12]
    G. E. Berendsen, L. Galan, J. Liq. Chromatogr.1, 561 (1978).Google Scholar
  13. [13]
    J. H. Knox, “High Performance Liquid Chromatography” Edinburgh, University Press, p. 49 (1977).Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1997

Authors and Affiliations

  • C. Delaurent
    • 1
  • V. Tomao
    • 1
  • A. M. Siouffi
    • 1
  1. 1.UMR Synthèse et Catalyse, Faculté des Sciences et Techniques de St JéromeUniversité d'Axis-Marseille IIIMarseille cedex 20France

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