Use of a keeper to enhance the recovery of volatile polycyclic aromatic hydrocarbons in HPLC analysis
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The recorveries of volatile polycyclic aromatic hydrocarbons in HPLC analysis may be enhanced if a long-chain alcohol, e.g. 1-hexanol or 1-octanol, is used as keeper in the solvent-evaporation step. Even under the harsh conditions of the vacuum-concentrator centrifuge, recoveries of more than 90% were achieved for 14 of the 15 PAH tested. The use of liquid-liquid extraction (LLE) eliminates the problem of adsorption of highly condensed aromatics by the wall of the sampling bottle. The combination of LLE with vacuum-controlled rotary evaporation and use of a keeper yielded overall recoveries between 50 and 130%, for most substances close to 100%, from tap water spiked with trace concentrations.
Key WordsColumn liquid chromatography Polycyclic aromatic hydrocarbons Keeper Extraction Recovery
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