Advertisement

Chromatographia

, 45:145 | Cite as

Determination of 8-hydroxyquinoline sulfate in tuberculin solutions by planar and high performance liquid chromatography

  • D. Agbaba
  • A. Mihajlovic
  • P. Beljanski
  • D. Zivanov-Stakic
  • S. Vladimirov
Originals

Summary

TLC and HPLC methods for the determination of the preservative, 8-hydroxyquinoline sulfate in PPD-T tuberculin solution were developed. The planar chromatography method involved separation of 8-hydroxyquinoline sulfate on a TLC plate using a butyl-acetate: formic acid: 2-propanol mobile phase, detection and quantitation by densitometric scanning. The HPLC method was on a LiChrosorb RP-18 column with acetonitrile-water (65:35 v/v) mobile phase, adjusted to pH 3.05 by phosphoric acid. Linearity, reproducibility and accuracy were found to be satisfactory. Under selected conditions, the limit of detection (LOD) of both methods was similar-about 25 ng.

Key Words

Thin-layer chromatography Column liquid chromatography PPD-tuberculin solution 8-Hydroxyquinoline sulfate-preservative 

References

  1. [1]
    The United States Pharmacopoeia 23rd Revision, The United States Pharmacopeial Convention, Rockville, MD, (1995), p. 2212.Google Scholar
  2. [2]
    Pharmacopeé Francaise IX, I partie, Commission nationale de Pharmacopee par l'ordre national des Pharmaciens, Oxyquinol (1976).Google Scholar
  3. [3]
    Pharmacopoea Helvetica, 7th ed., Département Fédèral del' interieur, Berne, Oxychinolini sulfas (1993).Google Scholar
  4. [4]
    Pharmacopoea URSS IX, 9th ed., Ministere de la Santé puplique de l'URSS, 1961, p. 114.Google Scholar
  5. [5]
    V. Mejstrik, L. Drzleva, Z. Sagnes, Chem. Prum.38 (7), 361 (1988).Google Scholar
  6. [6]
    S. S. Artemchenko, V. V. Petrenko, Otkrytiya Izobret27, 144 (1985).Google Scholar
  7. [7]
    S. S. Atemchenko, V. V. Petrenk, N. P. Zhovna, V. A. Tsilinko, Zh. Anal. Khim.40(4), 744 (1985).Google Scholar
  8. [8]
    N. P. Yavorśkii, E. M. Babich, E. P. Koreńkova, Farmatseut. Zh.19(4), 29 (1986).Google Scholar
  9. [9]
    K. Hajime, Y. Yamamura, Y. Tanimura, Y. Sartoh, F. Nakagawa, Z. Tamura, Chem. Pharm. Bull.31(1), 299 (1983).Google Scholar
  10. [10]
    Z. Chimel, Chem. Anal.31(1), 63 (1986).Google Scholar
  11. [11]
    K. Miura, H. Nakamura, H. Tanaka, Z. Tamura, J. Chromatogr.210, 536 (1981).CrossRefGoogle Scholar
  12. [12]
    E. J. Wojtowicz, J. Pharm. Sci.73, 1430 (1984)Google Scholar
  13. [13]
    I. Nagiel-Ostaszewski, M. T. Vavrek, J. H. Weisburger, Xenobiotica6(21), 751 (1991).CrossRefGoogle Scholar
  14. [14]
    N. De Kruijf, M. A. H. Rijk, L. A. Pranoto-Soetardhi, A. Schouten, J. Chromatogr.410, 395 (1987).CrossRefGoogle Scholar
  15. [15]
    Y. S. Chung, O. H. SengHo, K. W. Lee, Y. S. Yoon, N. S. Lee, Anal. Sci. Technol.3(1), 43 (1990).Google Scholar
  16. [16]
    W. Wardas, A. Pyko, M. Jedrzejczak, J. Planar chromatography —Mod. TLC.6(3), 238 (1993).Google Scholar
  17. [17]
    H. J. Lukas, T. E. Spratt, M. T. Vavrek, S. F. Roland, J. H. Weisburger, Cancer Res.47, 4407 (1989).Google Scholar
  18. [18]
    Clarke's Isolation and Identification of Drugs, The Pharmaceutical Press, London, 2 edn., 1986, p. 672.Google Scholar
  19. [19]
    H. R. Held, S. Landi, Bulletin WHO33, 395 (1965).Google Scholar
  20. [20]
    H. R. Held, S. Landi, J. Pharm. Sci.4(57), 605 (1968).Google Scholar
  21. [21]
    H. R. Held, S. Landi, H. Prvnick, Bulletin WHO38, 809 (1968).Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1997

Authors and Affiliations

  • D. Agbaba
    • 1
  • A. Mihajlovic
    • 2
  • P. Beljanski
    • 2
  • D. Zivanov-Stakic
    • 1
  • S. Vladimirov
    • 1
  1. 1.Department of Pharmaceutical ChemistryFaculty of PharmacyBelgradeSerbia (Yugoslavia)
  2. 2.Torlak-Institute of Immunology and VirologyBelgradeSerbia (Yugoslavia)

Personalised recommendations