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Chromatographia

, Volume 45, Issue 1, pp 52–58 | Cite as

A study of the behaviour of some new column materials in the chromatographic analysis ofCatharanthus alkaloids

  • G. Theodoridis
  • I. Papadoyannis
  • A. Hermans-Lokkerbol
  • R. Verpoorte
Originals

Summary

New column-packing materials specially designed for the HPLC analysis of basic compounds have been tested for the analysis ofCatharanthus alkaloids. Mobile phase optimization was performed for each column tested. The influence of mobile phase pH, nature and content of the organic modifier and salt concentration on retention, selectivity and resolution was studied. An important factor in the separation proved to be the pH of the eluent, because of the widely different pK a values of the analytes. Complete separation was easily achieved on ODS columns, but polymeric materials also gave acceptable results.

Key Words

Column liquid chromatography Catharanthus alkaloids New column-packing materials 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1997

Authors and Affiliations

  • G. Theodoridis
    • 1
    • 2
  • I. Papadoyannis
    • 1
  • A. Hermans-Lokkerbol
    • 2
  • R. Verpoorte
    • 2
  1. 1.Laboratory of Analytical Chemistry, Department of ChemistryAristotle UniversityThessalonikiGreece
  2. 2.Division of PharmacognosyGorlaeus LaboratoriesLeidenThe Netherlands

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