Anatoxin-a is a naturally occurring, potent neurotoxin produced by some species of blue-green algae. Release of anatoxin-a into water bodies on cell lysis is of concern to farmers, whose livestock may be affected by drinking contaminated water; to recreational users of reservoirs where a blue-green algal scum is present; and to water companies, who need to be certain that levels of anatoxin-a in the water distribution system present no threat to public health.
The present work describes the extraction and analysis of anatoxin-a from water samples. Extraction was performed using a polymeric resin solid-phase extraction cartridge, and analysis of the resulting extract was by reverse phase HPLC with UV detection. Data are presented describing the performance characteristics of the method, including limit of detection, precision, bias and recovery.
Key wordsColumn liquid chromatography Solid phase extraction Algal toxins Blue-green algae Drinking water and river water
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- H.-D. Park, M. F. Watanabe, in “Toxic Microcystis”, M. F. Watanabe, K.-I. Harada, W.W. Carmichael, H. Fujiki, eds., CRC Press, Boca Raton, 1996, p. 57.Google Scholar
- J. K. Fawell, H. A. James, “Toxins from Blue-Green Algae: Toxicological Assessment of Anatoxin-a and a Method for its Determination in Reservoir Water”, Report No. FR0434, Foundation for Water Research, Marlow, Bucks, UK, 1994.Google Scholar
- G. E. Fogg, W. D. P. Stewart, P. Fay, A. E. Walsby, “The Blue-Green Algae”, Academic Press, London, 1973.Google Scholar
- M. Ekman-Ekebom, M. Kauppi, K. Sivonen, M. Niemi, L. Lepistö, Environ. Toxicol. Water Quality7, 201 (1992).Google Scholar
- R. A. Smith, D. Lewis, Vet. Hum. Toxicol29, 153 (1987).Google Scholar
- J. Kiviranta, K. Sivonen, K. Lahti, R. Luukkainen, S. I. Niemelä, Arch. Hydrobiol.121, 281 (1991).Google Scholar
- H. A. James, C. Smith, A. Sutton, “The Persistence of Anatoxin-a in Reservoir Water”, Report No FR0427, Foundation for Water Research, Marlow, Bucks. UK. 1993.Google Scholar
- N. B. Astrachan, B. G. Archer, Environ. Sci. Res.20, 437 (1981).Google Scholar
- S. H. Wong, E. Hindin, J. AWWA74, 528 (1982).Google Scholar
- C. P. James, H. A. James, “An analytical method for Anatoxin-a, a blue-green algal neurotoxin, in reservoir water”, Report No. FR0363, Foundation for Water Research, Marlow, Bucks, UK. 1993.Google Scholar
- D. K. Stevens, R. I. Krieger, J. Anal. Toxicol.12, 126 (1988).Google Scholar
- T. M. Jefferies, G. Brammer, A. Zotou, P. A. Brough, T. Galagher, in “Detection Methods for Cyanobacterial Toxins”, G. A. Codd, T. M. Jefferies, C. W. Keevil, E. Potter, eds., The Royal Society of Chemistry Special Publication No. 149, 1994, p. 34.Google Scholar
- M. M. Ross, D. A. Kidwell, J. H. Callahan, J. Anal. Toxicol.13, 317 (1989).Google Scholar
- J. K. Fawell, H. A. James, “Toxins from Blue-Green Algae: Toxicological Assessment of Anatoxin-a and a Method for its Determination in Reservoir Water”, Report No. FR0434, Foundation for Water Research, Marlow, Bucks, UK, 1994, Appendix A.Google Scholar
- P. D. McDonald, E. S. P. Bouvier, eds. “Solid Phase Extraction, Applications Guide and Bibliography”, 6th edition, Waters, Milford, 1995.Google Scholar
- K. C. Van Horne, ed., “Sorbent Extraction Technology”, Analytichem International, Harbor City, 1990.Google Scholar
- G. C. Mattern, J. B. Louis, L. D. Rosen, J. Assoc. Off. Anal. Chem.74, 982 (1991).Google Scholar
- H. G. Janssen, R. Perkins, G. Hutchison, C. Fraundeau, Int. Environ. Technol.6, 14 (1996).Google Scholar
- R. V. Cheeseman, A. L. Wilson, “A Manual on Analytical Quality Control for the Water Industry”, Report No. NS30, Revised by M. J. Gardner, Water Research Centre, Medmenham, Bucks. UK. 1989.Google Scholar