Summary
A new bonded liquid-crystal stationary phase for high-performance liquid chromatography is presented. Synthesis entailed reaction of γ-benzyl-L-glutamate-N-carboxyanhydride with aminopropyl LiChrospher 100 NH2. The new stationary phase was characterized by solid-state13C NMR and elemental analysis. Its analytical properties were tested with polynuclear aromatic hydrocarbons (PAH) and phenols. PAH were chromatographed with hexane-chloroform mixtures as mobile phases and were eluted in order of increasing ring number. Total resolution of the 22 PAH studied was achieved by gradient elution. Phenols were chromatographed with a mixture of chloroform and methanol as mobile phase. Small amounts of methanol have a substantial effect on the separation of the phenols. Use of 0.5% (v/v) methanol enabled the separation of 19 phenols and phenol derivatives.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
A.G. Walton, J. Blackwell, Biopolymers, Academic Press, New York, 1973.
R.D.B. Frazer, T.P. MacRae, Conformation in Fibrous Proteins, Academic Press, New York, 1973.
J. Watanabe, S. Sasaki, I. Uematsu, Polym. J. (Tokyo)9, 451 (1977).
J. Watanabe, S. Sasaki, I. Uematsu, Polym. J. (Tokyo)9, 337 (1977).
J. Watanabe, K. Imai, R. Gehani, I. Uematsu, J. Polym. Sci. Polymer. Phys. Ed.19, 217 (1981).
J. Watanabe, I. Uematsu, Polymers,25, 698 (1984).
J. Watanabe, H. Ono, I. Uematsu, A. Abe, Macromol.18, 1141 (1985).
A. Meddour, J. Courtieu, W. Abdelhadi, S. Guermouche, M.H. Guermouche, Chromatographia43, 387 (1996).
A. Meddour, I. Canet, A. Loewenstein, J.M. Pechine, J. Courtieu, J. Am. Chem. Soc.,116, 9652 (1994).
I. Canet, J. Courtieu, A. Loewenstein, A. Meddour, J.M. Pechine, J. Am. Chem. Soc.117, 6520 (1995).
A. Meddour, P. Berdague, A. Hedli, J. Courtieu, P. Lesot. J. Am. Chem. Soc.119, 4502. (1997).
M. Jakubcova, A. Meddour, J.M. Pechine, A. Baklouti, J. Courtieu, J. Fluorine Chem.86, 149 (1997).
M.L.C.M. Oosterling, E. Willems, A.J. Schouten, Polymer36, 4463 (1995).
E. Dietz, N. Fery, K. Hamman, Angew. Makromol. Chem.35, 115 (1974).
L.C. Sander, S.A. wise, Anal. Chem.56, 504 (1984).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ferroukhi, O., Guermouche, S., Guermouche, M.H. et al. Separation of polynuclear aromatic hydrocarbons and phenols on a new chemically bonded phase for high-performance liquid chromatography. Chromatographia 51, 701–705 (2000). https://doi.org/10.1007/BF02505408
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02505408