Summary
An LC-MS/MS method has been developed for the sensitive determination of the anabolic compound zeranol, its epimer taleranol and the mycotoxins zearalenone, α-zearalenol and β-zearalenol in animal urine and meat samples. Sample preparation included extraction of meat samples and enzymatic digest of urine samples followed by solid phase extraction with RP-18 columns for sample clean-up. Mass spectrometric determination was carried out with an atmospheric pressure chemical ionisation interface (APCI) in the multi-reaction monitoring mode (MRM). Using the negative ion mode, a limit of detection between 0.1 and 0.5 ppb and a limit of determination between 0.5 and 1 ppb could be achieved. With zearalanone (ZAN) as internal standard, a linear range between 0.5 (1.0) and 100 ppb in urine samples (cow, pig) and between 1 and 100 ppb in meat samples (cow, calf, pig) could be established. Depending on the biological matrix and analyte, standard deviations were below 8.5%, with average recovery rates around 86% to 102% in spiked samples. The usefulness of the method developed was demonstrated via several contaminated cow and pig urine samples.
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References
N. A. Cole, D. P. Hutchson, J. B. McLaren, W. A. J. Phillips, J. Anim. Sci.58, 527 (1984).
A. F. Erasmuson, B. G. Scahill, D. M. West, J. Agric. Food Chem.42, 2721 (1994).
C. O. Miles, A. F. Erasmuson, A. L. Wilkins, N. R. Towers, B. L. Smith, I. Garthwaite, B. G. Scahill, R. P. Hansen, J. Agric. Food Chem.44, 3244 (1996).
D. G. Kennedy, S. A. Hewitt, J. D. G. McEvoy, J. W. Currie, A. Cannavan, W. J. Blanchflower, C. T. Elliot, Food Add. Contam.15, 393 (1998).
V. Beting, in V. Betina, Ed., Bioactive Molecules, Mycotoxins, Vol. 9, Elsevier, Amsterdam, 1989, p. 271.
J. D. Miller, H. L. Trenholm, Eds., Mycotoxins in Grain. Compounds Other than Aflatoxins, 2nd ed., Eagan Press, St. Paul, MN, 1997.
H. van Egmond, FAO Food and Nutrition Paper, advanced copy, p. 7.
K. E. Richardson, W. H. Hagler, C. J. Mirocha, J. Agric. Food Chem.33, 862 (1985).
M. Medina, N. Nagdy, J. Chromatogr.614, 315 (1993).
M. T. Liu, P. B. Ram, L. P. Hart, J. J. Petska, Appl. Environ. Microbiol.59, 1178 (1978).
R. Warner, B. P. Ram, L. P. Hart, J. J. Petska, J. Agric. Food Chem.34, (1986).
J. Plasencia, C. J. Mirocha, R. J. Pawlosky, J. F. Smith, J. Assoc. Off. Anal. Chem.73, 973 (1990).
L. A. van Ginkel, E. H. J. M. Jansen, R. W. Stephany, P. W. Zoontjes, P. L. W. J. Schwillens, H. J. van Rossum, T. Visser, J. Chromatogr.624, 389 (1992).
M. A. S. Marques, L. A. Lima, C. H. B. Bizarri, F. R. A. Neto, J. N. Cardoso, J. Anal. Toxicol.22, 367 (1998).
G. H. Ingerowski, H. -J. Stan, J. Environ. Pathol. Toxicol.2, 1173 (1979).
H. L. Kim, A. C. Ray, R. D. Stipanovic, J. Agric. Food Chem.34, 312 (1986).
V. Seidel, E. Poglits, K. Schiller, W. Lindner, J. Chromatogr.635, 227 (1993).
R. Schuhmacher, R. Krska, M. Grasserbauer, W. Edinger, H. Lew, Fresenius J. Anal. Chem.360, 241 (1998).
E. Rosenberg, R. Krska, R. Wissiack, V. Kmetov, R. Josephs, E. Razzazi, M. Grasserbauer, J. Chromatogr. A819, 277 (1998).
P. Zöllner, J. Jodlbauer, W. Lindner, J. Chromatogr. A858, 167 (1999).
P. Zöllner, D. Berner, J. Jodlbauer, W. Lindner, J. Chromatogr. B.738, 233 (2000).
R. P. Huopalathi, J. D. Henion, J. Liq. Chrom. Rel. Technol.19, 69 (1996).
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Jodlbauer, J., Zöllner, P. & Lindner, W. Determination of zeranol, taleranol, zearalenone, α- and β-zearalenol in urine and tissue by high-performance liquid chromatography-tandem mass spectrometry. Chromatographia 51, 681–687 (2000). https://doi.org/10.1007/BF02505405
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DOI: https://doi.org/10.1007/BF02505405