Prevalence of human papillomavirus DNA in cervical tissue. Retrospective analysis of 855 cervical biopsies
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The histopathologic features of 855 cervical biopsies were correlated with the presence of human papillomavirus DNA using in situ hybridization (ISH) with biotin labeled type specific probes for Human Papilloma Virus (HPV) types 6, 11, 16, 18, 31, 33 and 51. HPV-DNA was found in 18% (13/72) of cervical intraeptihelial neoplasia I (CIN I), 30% (35/115) of CIN II, 28% (57(206) of CIN III, in 84% (21/25) of flat condyloma and in 13% (15/112) of normal cervical tissue. HPV DNA was detectable in 11% (5/46) of cervical adenocarcinoma and in 21% (59/279) of squamous cell carcinoma (SCC) of the cervix. High risk HPV types were identified more often than low risk HPV types in CIN I, CIN II, CIN III and SCC. HPV type 16/18 predominates over HPV type 31/33/51 in CIN I, flat condyloma and in SCC. The prevalence of HPV was strongly associated with the grade of differentiation of SCC. It was identified in 59% (23/39) of well differentiated SCC, in 18% (25/142) of moderately differentiated and in 11% (11/98) of poorly differentiated SCC.
Key wordsHuman Papillomavirus In situ Hybridization CIN Squamous cell carcinoma of the cervix Grading
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- 1.Backe J, Mulfinger L, Ott M, Hillenbrand C, Roos T, Martius J (1996) Retrospective analysis of human papillomavirus DNA in cervical biopsies over ten years. Int J Fetomat Med: in pressGoogle Scholar
- 8.Duggan MA, Inoue M, McGregor SE, Stuart GCE, Morris S, Chang-Poon V, Schepansky A, Honore L (1994) A paired comprison of dot blot hybridization and PCR amplification for HPV testing of cervical scrapes interpreted as CIN 1. Eur J Gynaec Oncol 15:178–187Google Scholar
- 9.ENZO Diagnostics PathoGene DNA Probe Assay for Detection of HPV (1988) Enzo Diagnostics, New YorkGoogle Scholar
- 17.Johnson K (1995) Periodic health examination, 1995 update: 1. Screening for human papillomavirus infection in asymptomatic women. Can Med Assoc J 152:483–493Google Scholar
- 19.Kiviat NB, Koutsky LA, Critchlow CW, Galloway DA, Vernon DA, Peterson ML, McElhose PE, Pendras SJ, Stevens CE, Holmes KK (1990) Comparison of southern transfer hybridization and dot filter hybridization for detection of cervical human papillomavirus infection with types 6, 11, 16, 18, 31, 33 and 35. Am J Clin Pathol 94:561–565PubMedGoogle Scholar
- 23.Medas, Statistikprogramm für Medizin und Biowissenschaften, Grund C, Würzburg 1993Google Scholar
- 29.Reid R, Greenberg M, Jenson AB, Husain M, Willett J, Daoud Y, Temple G, Stanhope CR, Sherman AI, Phibbs GD, Lorincz AT (1987) Sexually transmitted papillomaviral infections: The anatomic distribution and pathologic grade of neoplastic lesions associated with differtient viral types. Am J Obstet Gynecol 156:212–222PubMedGoogle Scholar
- 32.Rosai J (1989) Female reproductive system: uterus-cervix. In: Ackerman’s surgical Pathology, 7ed, Vol II, The C.V. Mosby Company, Washington, p 1028–1029Google Scholar
- 42.World Health Organization (1992) Histopathologic classification of tumors and tumor-like lesions of the uterine cervix and vaginaGoogle Scholar